| Objective:in the experiment,Build acute soft tissue injury in rats by the classical hit method,Using Zheng's xinshang yao shui as a therapeutic drug,which is belong to Professor Zheng Huaixian's clinical experience prescrip.Select zhenggu shui for comparison,is not only a wide range of clinical use drug,also Efficacy and mechanism of action have been validated.using the external treatment,to observe the recovery of injured limbs in rats.To study activate blood circulation,remove blood stasis,activate collaterals,subduing swelling and alleviating pain-the efficacy of zheng's xinshangyao shui,is detection by the serum IL-1 beta and histomorphology.the research provides a theoretical reference for clinical treatment of acute soft tissue injury,Verify its therapeutic effect and mechanism of curative effect.In order to Zheng's department of traumatology prescription system to provide a scientific basis for clinical application.Methods:80 male SD rats were selected from March years old,after the success of the model,72 rats were weighed and recorded.then 72 rats were numbered andrandomly divided them into there groups,that A-experience group(zheng's xinshangyao shui)?B-pharmic group(zhenggu shui)?C-blank control group(normal saline).The three groups were randomly divided into 4 time observation point groups(1?3?7?10,n=6).The experimental group, drug control group,blank control group were each salined the Zheng's xinshangyao shui, zhenggu shui,normal saline with2 h,every half hour to give the medicine 0.4ml,twice in a day, a total of 10 days,8-10 morning dressing time, 18-20 afternoon dressing time.At each observation point:weight of corresponding rats,evaluation of soft tissue injury syndrome,observation of tissue morphology and score it under light microscope,enzyme linked immunosorbent assay for detection of IL-1 beta in rat serum,using statistical software(SPSS20.0) for the analysis of the selected data processing.Result:(1)Weight result:in the experiment,the weight were no significant differences in zheng's xinshangyao shui group, zhenggu shui group,normal saline group between different time points(P>0.05).(2)Soft tissue injury syndrome index:The soft tissue injury syndrome index number reached the peak in the first day,then the index showed declining trend. the numerical value in each group were comparable, no significant difference in the first day(P>0.05).In the third day and seventh day,Zheng's xinshangyao shui group, zhenggu shui group' numerical value were close and no significant difference(P>0.05),Zheng's xinshangyao shui group and zhenggu shui group had significant difference with normal saline group.In the tenth day,Zheng's xinshangyao shui group and zhenggu shui group's numerical value were below the blank control group's.No significant diverse between Zheng's xinshangyao shui group and zhenggu shui group(P>0.05),but Zheng's xinshangyao shui group?zhenggu shui group compared with the normal saline group,there was significant difference.(3)Histological observation:Over time,tissue edema in each group,Inflammatory cell infiltration decreased,Showing a tendency to reduce the inflammatory response,Zheng's xinshangyao shui group and zhenggu shui group improved more obviously than the saline group.The change tendency of the three groups of rats under microscope were similar.In the first day,the three groups numerical value were similar, reached the highest peak and no significant difference among the three(P>0.05).In the third, seventh, tenth day,Zheng's xinshangyao shui group ?zhenggu shui group?normal saline group's numerical value were reduced to a state of decline.among them,zhenggu shui group's numerical value were lowest,normal saline group's were highest.Zheng's xinshangyao shui group and blankcontrol group comparing the results morphology under the microscope had significant difference(P<0.05),zhenggu shui group and blank control group compared with significant difference(P<0.05).Zheng's xinshangyao shui group and zhenggu shui group compared with no significant difference(P> 0.05).(4)Serum IL-1 beta( pg/ml):comparison among groups:the first day,zhenggu shui group?Zheng's xinshangyao shui group compared with normal saline group had no significant difference(P>0.05),there was no significant difference between zhenggu shui group and Zheng's xinshangyao shui group(P>0.05);The third day,Zheng's xinshangyao shui group and zhenggu shui group had no significant difference(P>0.05),between Zheng's xinshangyao shui group and normal saline group, there were significant difference(P<0.05);The seventh day,Zheng's xinshangyao shui group, zhenggu shui group and normal saline group showed a significant difference among them(P<0.05);The tenth day,among Zheng's xinshangyao shui group, zhenggu shui group and normal saline group had a significant difference(P<0.05);Comparison within groups:Zheng's xinshangyao shui group decreased in third days after the peak,the third day and first day?the Seventh day and third day?The tenth day and the Seventh day all had significant difference(P<0.05);zhenggu shui group,in the test,there were significant differences in each time point(P<0.05);normal saline group,in four time phase point, there were significant differences among them(P<0.05);Conclusion:For acute soft tissue injury,Zheng's xinshangyao shui can inhibit the secretion of IL-1 beta in serum during acute inflammation stage,In order to achieve anti-inflammatory, promote the repair of soft tissue injury. |
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