Study On The Action Mechanism Of Peach Kernel On Bleomycin Induced Interstitial Lung Disease In Rats

Objective: Interstitial lung disease rat model was established by animal experiment in this study.we observe and analyse the act on Peach kernel of interstitial lung disease in rats and its mechanism from pathology, oxidative stress, cell factor and gene expression perspective.Methods: This experiment chooses the SPF level male Wistar 70 rats.After adaptive feeding 7days, randomly divided into 3 groups: The blank group(group A), model group(group B) and high density peach group(group R), concentration of peach group(group S), low concentration of peach group(group T), pirfenidone group(group X) and combined group(group Z). After adaptive feeding 3 days, we use the method of intratracheal bleomycin to establish interstitial lung disease rat model and give the corresponding drug intragastric administration. The rats were sacrificed at twenty-eighth days, the pathological changes in lung tissue of rats in each group were observed under light microscope. the expression level of SOD,MDA of lung tissue was detected in Chemiluminescence method. expression levels of IL-8, type I collagen of abdominal aorta in rats was detected in ELISA method. the expression level of VEGF-mRNA, TGF- beta 1 of lung tissue was detected in PCR method. SPSS 17 software was used for data processing.Results and conclusion: Peach kernel can treat interstitial lung disease model rats from a variety of ways, Some indicators of treatment outcome related to dose of peach kernel. The mechanism of action of peach kernel for interstitial lung disease in rats may be related toInhibition of inflammatory response, Correcting the oxidative stress state,improving airway remodeling, inhibiting the neovascularization...