Research On Logical Gates Based On Molecular Beacon

DNA computing is a new biological computing discipline after the electronic computing. With the scale of electronic computer gradually approaching to the bottleneck, and electronic computer can not solve NP problems and NP-hard problem, so the development of various types of computers began to receive attention. The DNA computing with the advantages for the capacity of large memory, and the high degree of parallelism, etc., can solve math problems in some of the NP. In recent years, more and more researchers began to focus on DNA computing problem and solved difficult issues which can not be solved in the electronic calculation by using DNA molecules, and it provides a bright future for the development of new biological computer.As a fundamental part of electronic computer, the logic gate is a prerequisite for carrying out four preconditions operation. Also in the process for the development of DNA computer, as the basic computing architecture, the logical gates are also preconditions for solving more complex problems. In recent years, people began to use the characteristics of complementary base pairing that DNA structure has to build different molecular logic gates. For the study of molecular logical gates, many scholars have proposed the paste model, and surface model to solve the problem of molecular logical gates. These methods are mainly according to the state of a single or double-stranded DNA structure to realize the decision of logical results. In the testing of experimental result, which mainly uses the DNA sequencing to determine the true value of the logic, it is prone to error generated by wrong hybridization, and the sensitivity is not high. This paper combines the flexibility of hybridization that DNA has, and uses the characteristics of molecular beacons, by designing the special structure of DNA, making molecular beacons as input signals and the DNA structure as the basic structure, to achieve the configuration of logical gates.The innovation of this method is that it makes molecular beacons into the design of logical gates, and achieves the determination of logical results mainly by the intensity of the fluorescence in the reaction solution. It is not present in the prior design.of molecular logical circuit, which broadens the scope of input signals in logical circuit configured with DNA molecules. The detection method is sensitive, simple. Its requirement about base mismatches is not so strict, so it reduces the impact to experiments that competitive hybridization brought. However, the further study which needs improvement is that, as the hybridized reactions in solution, and since the pre-reaction solution contains fluorescence, the error that the insufficient reaction and error hybridization brings still exists.