Research Of Molecular Calculation Model Based On DNAzyme Regulation

With the rapid development of science and technology,traditional computing is unable to meet the increasingly complex scientific problems.Is expected to break Moore's law and,therefore,the breakthrough tradition based on the silicon technology including quantum computing,photon calculation,nano calculation and calculation of creatures,including a new revolutionary unconventional computing arises at the historic moment,the focus of governments attention and funding.This paper combined with the frontier hot spots in the field of self-assembly technology,DNA fluorescence probe technology,strand displacement,around the multiple overlapping fields such as DNA computing and fluorescent molecular detection,at the same time use of high performance computer,and design software such as NUPACK for nano calculation based on DNA molecular computing model of DNAzyme regulation research from multiple perspectives.First,molecular calculation logic gate based on the self-assembly of AuNPs.In this molecular computing model,we have successfully designed and implemented a programmable strategy that can control the combination of AuNPs and specific DNA structures through multiple DNA input signal strands.The input operation was performed by adding the corresponding DNA strand,and the output signal was confirmed by agarose gel electrophoresis.This strategy uses strand displacement principle and proximity effect to realize various logic gate functions through the regulation of different DNA strands and the characteristics of cascade.(YES gate,the two levels YES gate and OR gate).In addition,in order to further explore cascade circuits,we have established a more complex catalytic coupling system(AND gate).Second,research of molecular calculation model based on DNAzyme regulation.Herein,we developed a system of cascading DNA logic gates regulated by DNAzymes.In the computing system,we combining the two mechanisms:DNAzyme catalysis and entropy-driven strand displacement.In our design,DNAzyme plays multiple roles as both an input signal and an output reporter.Firstly,DNAzyme triggers the initial substrate into active DNA substrates.Subsequently,the entropy-driven favorability is facilitated by a DNA catalyst,thus triggering the strand displacement to generate a new product of DNAzyme.With the help of entropy-driven strand displacement,the input DNA catalyst can be regenerated to trigger an another output DNAzyme product,which can then act as input to drive the next downstream targets,thus realizing a DNAzyme-based cascading catalytic circuit.In this study,a series of "logic gates"(YES,OR and AND)are achieved.Moreover,a two-layer cascading circuit and a feedback self-catalysis logic circuit were also established.The results were confirmed via native polyacrylamide gel electrophoresis(PAGE)and fluorescence detection.