| Study Background and Objective: Chronic atrophic gastritis is gastric glands atrophy, characterized by destruction of a common digestive disease. In 1978 WHO listed it as the precancerous condition of gastric cancer, and on this basis, there is associated with incomplete intestinal metaplasia and moderate to severe dysplasia were considered as precancerous lesions. It is seriously endangering people's lives and health. Therefore, the active treatment of their cancer prevention CAG is to reduce the incidence of gastric cancer an effective means of. Western medicine on the CAG no effective control methods, in medicine has some effect on CAG. Therefore, to explore the mechanism of CAG CAG cancer research and cancer intervention blocking of great significance for the primary prevention .Gastrin levels in peripheral blood by testing and observation of gastric mucosa and pathological changes in the expression of Bc1-2 protein to study the pathogenesis of CAG place to further explore the Chinese Lilac Weiling mixture in the treatment of the mechanism of CAG.Methods: Adoption of healthy, sexually mature, clean Wistar 60 rats, half male and a female, all with a commanding points caged male and female, were randomly divided into 5 groups: Normal control group, model control group, cloves, Weiling mixture of low, medium and high dose group, 12 in each group. control group was fed with normal saline, model group and experimental group were fed with sodium salicylate, a free drink ammonia.2months after the modeling ,in addition to the model control group and blank control group ,other groups were given corresponding doses of clove mixture in the stomach gavage for 6 weeks .Blood extract eye method, isolated serum, Gastrin kit contents.Profile control stomach drawn, Detected Gastric juice with a PH test paper . Observed under light microscope morphological changes of gastric mucosal cells; Gastric mucosa detected by immunohistochemistry the expression of Bcl-2 protein. Experimental data applications SPSS16.0 statistical software for data processing. Compared among groups using single factor analysis of variance. When homogeneity of variance method used LSD, Heterogeneity of variance Dunnett's method when used, Between the two groups using t test, compared among groups using chi-square test rate..Results: Serum gastrin in the model group were lower than the control group(P<0.01),The PH value of gastric juice model control group, Bcl-2 expression in gastric mucosa cells was significantly higher than the normal control group(P<0.01). Clove Wei ling mixture of serum gastrin levels in all treatment groups increased. PH value of gastric juice, gastric mucosal expression of Bc1-2 protein decreased. With the model group significantly different(P<0.01)Conclusion: Chronic atrophic gastritis rats PH value of gastric juice, gastric mucosal expression of Bc1-2 protein increased, decrease the secretion of gastrin. Showed that the incidence of chronic atrophic gastritis may be related to the lack of gastrin and gastric high-Bc1-2 protein expression. The degree of gastric mucosal atrophy the number of G cells was correlated. Changes in G cell number or changes in secretion of gastrin secretion could lead to change. Bc1-2 gene is important to inhibit apoptosis gene, Bc1-2 protein overexpression and gastric cancer was a positive correlation between the occurrence of. Bcl-2 protein over-expression under the action of harmful factors makes the damaged cells reduced apoptosis, cell life is extended, If further subjected to mutation, the mutant gene accumulated to a certain extent, forming tumors. Clove Wei ling mixture in varying degrees in all treatment groups could increase the secretion of gastrin, reduce Bcl-2 protein expression. hyperplasia of the gastric mucosal cells inhibit. Which Clove Wei ling mixture of large, medium dose group the most obvious role(P<0.01). Description of the drug in the treatment of CAG play a rat model the effect of Chinese medicine treatment for CAG to provide a more powerful evidence. |
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