Effect Of Clove Weiling Mixture On The Quantity Of VEGF And MDA In Rats Model Of ChronicAtrophic Gastritis

Study Backgroud and Objective:Chronic atrophic Gastritis (CAG) is a frequently-occurring disease of digestive system, common diseases。It is the gastric mucosal changes have occurred in chronic atrophic gastritis, often accompanied by intestinal metaplasia, in Chinese medicine, "stoma-chache" "fullness" and other areas to long course of disease, disease complex, has repeatedly made as a feature. The main pathological changes of gastric mucosa is thin, glandular atrophy, intestinal metaplasia and polyps easy to form, is considered one of gastric precancerous lesions. CAG is also aggressive treatment to prevent cancer, reduce the incidence of gastric cancer effective means. Over the years, has been digested in the field of research.At present, the cause of modern medicine on the CAG not yet been fully clarified, and treatment is also limited to Hp infection and dyspeptic symptomatic treatment, the lack of specific and effective treatment. In recent years, Chinese medicine treatment of chronic atrophic gastritis and accumulated much experience. Use of Chinese medicine treatment of this disease, traditional Chinese medicine theory as a guide, combined with TCM syndrome differentiation, flexible addition and subtraction drug toxicity, has more advantages.This study to detect effect of Clove wei ling mixture on the quantity of MDA and expression of VEGF in rats model of Chronic atrophic gastritis.Material and Method:Sixty Wistar rats were randomly divided into five groups:normal control group, model control group, Clore wei ling mixture low—dose group, Clore wei ling mixture middle—dose group and Clore wei ling mixture high—dose group. Except normal control group rats, CAG model was established in other rats by synthetical method:sodium salicylic was given once a day, and ammonia was drink freely etal, all the factors were given for two monthes. When the model was established successfully, Except normal control group rats and model control group, others rats were treated with different dose medicines, after six weeks, all the rats were sacrificed. Their gastric juice was collect to detect PH, their blood serum was collect to detect MDA by chemic method, their stomaches were collect to detect VEGF by immunohistochemistry methods and detect the histologic mucosa changes by microscopy.Results:Model group and normal control group, a significant increase in gastric PH value (P<0.01), MDA levels were significantly higher (P< 0.01), VEGF expression was significantly higher (P< 0.01), Gastric mucosa lesions significantly. Clore wei ling mixture in the PH value of the treatment group, MDA levels, VEGF expression index decreased, and the model control group there was a significant difference (P< 0.01)。Indicators between treatment groups were significantly different(P<0.01或P<0.05)。Conclusion:The Clore wei ling mixture on the CAG have a therapeutic effect in rats. Pathological morphology, can increase the gastric mucosal thickness, eliminating gastric inflammation, improve or even reverse gastric atrophy, intestinal metaplasia and atypical hyperplasia pathology. In the biochemical indicators, clove mixture in gastric juice can reduce the PH value, reduce lipid peroxidation metabolites MDA, inhibition of VEGF expression. May be by eliminating the oxygen free radicals, reduce or block the organization of lipid peroxidation, inhibits the proliferation of such over-the role of gastric mucosa to achieve the purpose of treatment.