Observation On The Anesthetic Effects Of α-Chloralose And Dexmedetomidine In Rats With Different Administration Methods

At present,a variety of anesthetic drugs have been used in the anesthesia of rats in clinical and scientific research,but these drugs still have some shortcomings,such as the toxic side effects of chloral hydrate,poor controllability during pentobarbital anesthesia,and isoflurane These shortcomings may have a certain impact on the smooth progress of scientific research and teaching.Oral anesthesia and intrarectal anesthesia have the advantages of reducing animal vigilance,reducing stress response,and convenient operation in veterinary clinical applications.Therefore,they have certain significance in scientific research and teaching experiments in rats.In this experiment,rats were anesthetized with α-chloralose + dexmedetomidine by oral anesthesia and intrarectal anesthesia.The anesthesia effects of the two anesthesia methods on rats were monitored and the feasibility of the two anesthesia methods was evaluated.And safety,lay the foundation for better clinical application of α-chloralose + dexmedetomidine for mucosal anesthesia in rats.Test content:(1)According to the results of the preliminary experiment,the anesthetics were divided into four groups according to the dose gradient,and the doses of each group were administered to the rats by oral gavage and rectal perfusion.The anesthesia induction period,maintenance period and The time of the recovery period,and the anesthesia effect score of the two administration methods.(2)Monitor the basic physiological indicators of rats at different doses and time points under the two modes of anesthesia:body temperature,respiratory rate,heart rate,mean arterial pressure,blood oxygen saturation,and screen out the two modes of administration The respective appropriate anesthesia dose.(3)Perform laboratory safety tests on appropriate doses:blood routine indexes,liver function and renal function indexes of rats before anesthesia,neutralization,and 24 hours after administration.Verify whether the dose will cause substantial damage and functional changes to the animal body.Test results:(1)Compared with the two methods of oral anesthesia and intrarectal anesthesia in different administration methods,the drug is not easy to flow out during oral anesthesia,the dosage of anesthetic is more accurate,and the anesthesia can be maintained for up to 2 hours.The average induction time of intrarectal anesthesia is reduced by about 10 minutes compared with oral anesthesia,and the anesthesia score can be maintained greater than 7 within 10-80 minutes after administration.(2)Among the test doses of oral administration,the dosage of 16 mg/kg ofα-chloralose + 0.6 mg/kg of dexmedetomidine has a good anesthetic effect,and the T drops to35.90 ± 0.39℃,RR The lowest was 32.3 ± 3.82 times/min,and the HR was 197.5 ± 9.43 times/min.There was no significant change in MAP;Sp O2 remained above 93%.In each group administered intrarectally,the dose of 13 mg/kg of α-chloralose + 0.6 mg/kg of dexmedetomidine had better anesthesia effects on rats,and compared with the basic physiological indicators of the body In the high-dose group,the effect was small,with T as low as 37.00 ± 0.23°C,RR as low as 40.7 ± 4.65times/min,HR 217.6 ± 9.43 times/min,and MAP did not change significantly;Sp O2 remained above 93%.(3)The test results of liver function and kidney function showed that all indexes of rats did not undergo significant changes beyond the normal range.After 24 hours,all blood indexes returned to normal,which was not significantly different from before the test.Through the clinical trials of castration in male rats,it is proved that this compound medication has a good anesthesia effect on rats.There is no awakening phenomenon in the rats during the operation.The analgesia,sedation and muscle relaxation effects are ideal and balanced,and the postoperative recovery is good.Based on the above test results :(1)The oral anesthesia effect of α-chloralose 16 mg/kg +dexmedetomidine 0.6 mg/kg is stable and the anesthesia is maintained for a long time.(2)The induction period of intrarectal anesthesia for α-chloralose 13 mg/kg + dexmedetomidine 0.6 mg/kg is short,and the irritation to rats is small,and the anesthesia recovery period is short.(3)The compound medication did not cause significant changes in the blood indicators of the rat’s body,did not cause changes to the physiological functions of the rats,did not cause damage to the liver and kidney functions of the body,high safety,and satisfies simple scientific research and teaching work in rats Needs.