Research On Molecular Computing Model Based On Enzyme

Electronic computers have profoundly changed the lives of human beings.However,due to the limitations of Moore's Law,scientists began to search for new computing models.And molecular calculations and quantum calculations came into being.However,molecular computing has become a hot topic of new computing because of its high computational potential including high storage,smallness,and high parallelism.DNA molecules are the main tools for molecular computing,and enzyme can react with the DNA molecules.Thus,molecular logic gates and molecular circuits are the basis of molecular computing.Molecular logic gates and molecular circuits that combine with enzyme have both biological significance and important research direction in the field of molecular computing.The research of this paper is to use DNA molecules and enzyme(nicking enzyme)to design the DNA molecular logic gates and DNA molecular circuits by combining biochemical techniques such as hybridization chain reaction and strand displacement.The specific work is as follows:1.Based on the hybridization chain reaction(HCR),the "YES" molecular logic gate,"AND" molecular logic gate,and the "OR" molecular logic gate with the nicking enzyme as input were designed.In this system,there are two kinds of logic inputs including single-enzyme logic input and multi-enzyme logic input.The logic input serves to control the initiation of the hybridization chain reaction,and the logic logic output of this molecular logic gate is the generation of the hybridization chain reaction.Then the results were analyzed by gel electrophoresis,which verified the correctness and feasibility of these molecular logic gates.2.After the molecular logic gates,the deeper research of DNA computing was conducted.Based on the strand displacement reaction and nicking enzyme,a DNA molecular circuit combining enzyme-catalyzed reaction with non-enzyme catalyzed reaction which can perform multiple cycles of reactions was designed.The entire molecular circuit is co-regulated by nicking enzyme and DNA strands took part on the non-enzyme catalyzed reaction.Furthermore,base on single-enzyme-controlled first-level molecular circuits,more complex structural molecular circuits such as multi-enzyme-controlled first-order molecular circuits and single enzyme-controlled cascade molecular circuits were also designed.After that,the fluorescent probe and gel electrophoresis were used for analysis to verify the correctness and feasibility of the molecular circuit computing models.In summary,this study designed DNA molecular logic gates and DNA molecular circuits based on enzyme and constructed different molecular computing models successfully.These computing models have feasibility.Combined the biological property of enzyme,these modelds provide some ideas for the field of DNA molecules computing and lay the foundation for subsequent research...