| Objectives:Through the observation of tumor volume of warming moxibustion on the rat of tumor bearing model,and the inhibition rate of tumor metastasis,the content of vascular endothelial growth correlative factors and the general effects,to explore theeffect of moxibustion on the expression of gastric cancer in rats EGF and VEGF,which used to cut off the tumor growth by the ways of antitumor new blood vessels,it is used to prove effect of Moxibustion in cancer prevention,at the same time,to expose the tumorinhibition mechanism,Finally,it can help to broaden the new ideas for the study of tumor prevention and treatment of moxibustion.Methods:50 SPF grade healthy SD rats were randomly divided into 5 groups,namely,normal group,sham operation group,model group,moxibustion group and infrared group.The transplanted gastric cancer model was prepared by gastric tissue submucosa transplantation.After biopsy,each group received corresponding treatment for 21 days.At the same time,recording the response from each activity of rats,mental state,fur color,urinate shit and so on.After the treatment,the rats were killed after the blood was taken from the neck.And the tumor was removed from the stomach,measure the weight and volumeto count the tumor suppressor rate,and test the expression of EGF and VEGF in the gastric tissue by ELISA.Results:(1)General situation:the three groups of rats which copied tumor in the later period of experiment,it had found that the rates of model group felt depressed and sleepy,and their stools are dry and watery normally,even that they responsed slowly.However,the thin degree of big rats in moxibustion and infrared group is much better than model group.The big rats' weight of model group is less than the normal group,the sham operation group and moxibustion group(P<0.05)respectively.There was no significant differences among normal group,sham operation group and moxibustion group.(2)The situation of gastric tumor volume inhibition rate and cancer cells transfer:the volume of moxibustion treatment group and infrared irradiation group is less than gastric cancer model group(P<0.05),.Among that thetumor growth inhibition rates of moxibustion treatment group and infrared irradiation group were 41.89%and 28.09%respectively.The numbers of gastric cancer cells transfer were less than model group and infrared irradiation group.(3)The content of EGF in gastric tissue:there was significant EGF content differences infrared irradiation group which compared with gastric cancer model group,normal group,the sham operation group,and the EGF content of gastric cancer model was less than other groups.The differences have the meaning of statics(P<0.05).Normally,there was no significant difference among the normal group,the sham operation group,the moxibustion treatment group and the infrared irradiation group(P>0.05).(4)Gastric tissue VEGF content:there was significant VEGF content differences infrared irradiation group which compared with gastric cancer model group,normal group,the sham operation group,and the EGF content of gastric cancer model was more than other groups.(P<0.05).There was no significant difference among the normal group,the sham operation group,the moxibustion treatment group and the infrared irradiation group(P>0.05).Conclusion:(1)Warming moxibustion can inhibit the rapid proliferation of tumor tissuc,and there is relative specificity of acupoints in tumor volum rat and inhabition cancer transfer among Zusanli(ST36),Zhongwan(RN12),Guan Yuan(RN4),Pishu(BL20),Weishu(BL21)Point.(2)The expression of EGF and VEGF in normal tissues is low,while VEGF in gastric carcinoma is over-expression,and EGF are very low.(3)The mechanism of warming moxibustion against tumor is probably by adjusting the content of EGF and VEGF to accelerate the necrosis of malignant tissue and achieve anti-tumor effect. |
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