Research On Warming And Promoting Effect From Moxibustion And Correlation Of Trpv1

Background and Objective:Moxibustion medicine clinical treatment and prevention is the commonly used method health care, the clinical curative effect has been widely recognized. Modern research proves the moxibustion can adjust the viscera function, promote metabolism, improve the immune function, adjust the circulation, breath, digestive and neuroendocrine system function role of the human body, especially has significant advantage in the treatment of chronic disease prevention and health care abilities. Previous studies pay more attention to moxibustion clinical disease treatment effect to the disease, the mechanism of moxibustion therapy was not very clear. Recently, the researchers will turn the focus on researching the role of moxibustion itself characteristic, moxibustion biological physical characteristics have more understanding. But the stimulation of moxibustion characteristics and treatment effect of communication between research also insufficiently thorough, the research on moxibustion work mechanism is also not clear. Warm stimulation is moxibustion stimulation characteristics, the blood circulation is the moxibustion important therapeutic effect, the skin is moxibustion direct operation object. Recent neural biology research shows that the skin temperature distribution of receptors, has a variety of neuroendocrine function, the skin of these biological function, especially the temperature sensor TRP family transient receptor potential vanilloidsubfamily member1(TRPV1) may be related of warming and promoting effect from moxibustion starting mechanism. This study attempts to research the warming and promoting effect from the moxibustion stimulation associated with TRPV1activation, in order to find the possible starting mechanism of warming and promoting effect from moxibustion.Method:The study has two parts of the theoretical and experimental research. In the basis of the research on the theory, three aspects of the research:1,Study on the knowledge of the role of moxibustion stimulate characteristics, moxibustion treatment effect of the adjustable Pi,Luo,Mai, which is recorded in the ancient books before the qing dynasty;2,Summarizing the research progress in modern moxibustion clinical and experimental research of the literature, the characteristics of the warm stimulation, the role of the blood; and the response of the local sticks and moxibustion warming and promoting effect;3-. Analyze moxibustion warming and promoting effect and the possible mechanism. associated with new understanding of modern neural biology recent research and moxibustion stimulate characteristics and treatment effect. With experimental study as the main body, which on the basis of theory research, including three aspects of experiments.1-. By using the mature electrophysiology method, comparing different temperature (38℃,46℃) of moxa-stick moxibustion to hot stimulus bradycardia rat models of cardiac function, blood flow circulation influence, through the multi-channel physiological signal monitoring data acquisition instrument;2,By using more advanced small animals living imaging technology, observe different temperature (38℃,46℃) of moxa-stick moxibustion to the wild type heat stimulus propranolol mice on the distribution of the metabolism, and compares the difference;3,Through the organization of mathematical morphology method, comparing different temperature (38℃,46℃) of moxa-stick moxibustion to the wild type heat stimulus normal mice and TRPVl-/-mice point sticks; morphology and the function of the Shenque point local skin effect. By using HE dyeing method observation the change of the structure of moxibustion warm to stimulate Shenque point local skin tissue, and using Toluidine Blue staining method observation moxibustion warm to stimulate Shenque point local mast cells number and the influence of the form, the use of immunohistochemical method observation moxibustion warm to stimulate Shenque point local CGRP, SP, VIP, the difference of neuropeptide expression, and on this basis, further analysis the relationship among with the moxibustion warm stimulation, local change and TRPV1, and discusses the possible starting mechanism of moxibustion warming and promoting effect.Result:theoretical research:1,Ancient dortors accumulated a wealth of knowledge, hot as fire moxibustion stimulation characteristics, the Blood circulatin is the important effect of moxibustion and the characteristics of the Pi,Luo,Mai;2,Modern researchers have a large amount of research on the temperature difference of moxibustion, moxibustion stimulate, therapy bank Qi and activate Blood circulation and function of sticks; local effects and moxibustion mechanism and so on;3,Modern neurobiology provides new understanding knowledge base from skin biology function to promote the moxibustion temperature effect starting mechanism.experimental research:1, Different temperature (38℃,46℃) of moxa-stick moxibustion to hot stimulus bradycardia model of rats HR, MAP, LVSP,+dp/dtmax, t-dp/dtmax are improved;2,46℃moxa-stick moxibustion to hot stimulus bradycardia rats HR, MAP, LVSP,+dp/dtmax, t-dp/dtmax than38℃-the influence of thermal stimulate rate difference dividing article very significantly statistical significance (P<0.01);3,Different temperature (38℃,46℃) of moxa-stick moxibustion to hot stimulus mice propranolol metabolism all affect the distribution;4,46℃moxa-stick moxibustion to hot stimulus mice propranolol metabolism is38℃--the influence of thermal stimulation to speed up sticks article;5,Shenque point local skin structure form of Wild type mice is different from TRPV1-/-mice; 6,Different temperature of the hot stimulus and moxa-stick moxibustion medications to stimulate different degree of change in mice point of Shenque local skin morphological structure,46℃moxa-stick moxibustion in mice hot article stimulate Shenque point sticks; local skin organization form changes in the most;7,Different temperature of the hot stimulus and moxa-stick moxibustion medications to stimulate different degree of change in the number and form of Shenque point local mast cells, compared with other group interference,46℃moxa-stick moxibustion boost to mice hot; Shenque point local sticks the increasing number of mast cell number, a pronounced mast cells degranulation phenomenon;8,46℃moxa-stick moxibustion heat affected the mice sticks stimulation; local skin tissue dividing CGRP, SP, VIP neuropeptide expression.Conclusion:theoretical research:1,The fire, hot, is the characteristics of moxibustion stimulation, moxibustion temperature difference in the stimulus is the premise of moxibustion warming and promoting effect;2,Unobstructed the Mai, activate the Qi and Blood circulation, is the important effect of moxibustion;3,Local skin is moxibustion direct operate parts, the characteristic of Pi and Luo is the basis of moxibustion effective role;4,The local effect is related to the mechanism of moxibustion;5,The biological function of skin is the scientific basis of moxibustion warming and promoting effect mechanism.experimental research:1,Use of moxibustion the characteristic of warm stimulation, the effect of improving heart function by46℃(which can activate TRPV1) stimulus is different from38℃warm stimulation;2,Activate the Qi and Blood circulation is the main moxibustion warming and promoting treatment effect, the effect of unobstructed the Mai by46℃(which can activate TRPV1) stimulus is different from38℃warm stimulation;3,Local is moxibustion direct operate parts, the local effect by46℃(which can activate TRPV1) stimulus is different from38℃warm stimulation;4,The local effect of different temperature heat of moxibustion stimulating is associated with the adjustment of whole function;5,The starting mechanism of moxibustion warming and promoting effect is based on the local skin biological function6,Moxibustion wanning and promoting effect is related to TRPV1, moxibustion may work by activating TRPV1trigger skin neurogenic inflammation play a role.