Research Of Flow System Analysis Instrument Detector Based On SPR

Flow system analysis instrument is one of the most popular analytical testing instruments, detector is one of its key parts, and detector's performance influence the flow system analysis instrument's performance directly. Traditional detectors usually have limitations when they are used in flow system analysis instrument. Developing a universal, sensitive, easy-taking and low cost detector has strong practical values for the testing of trace substances and also is a hot research area of analytical testing instruments. Surface plasmon resonance (SPR) detection technique is a new photoelectric detection technique, and it has characteristics as high sensitivity, label-free, fast testing, simplicity, and low cost, and it has been widly used in many fields. This paper developed a new detector based on SPR technique, and experiments were taken about this SPR detector. The main research and innovative points include:1) A new SPR detector including micro flow cell, SPR sensor and circuit was build.2) SPR detector's workstation software was developed using visual C++ and database technique, and it was used for data collecting, processing, analysing, displaying and saving.3) Research of data processing methods of SPR spectrum was developed. In order to improve the existing 1st moment analysis methods of dynamic baseline, a kind of 1st moment analysis method of dynamic baseline based on amplitude was proposed. The baseline of this method adjusts dynamically according to the minimum value. The results show 1st moment analysis method of dynamic baseline based on amplitude can reduce not only the influence of fluctuations in optical power on SPR spectrum signals but also the calculating time.4) A kind of flow system detection instrument including high pressure pump, sampler and SPR detector was build. Using this instrument, the flow rate's influence on SPR detector was studied; noise and drift of SPR detector were tested. Using this instrument, salts, carbohydrates, inorganic small moleculars and their detection limits were detected.5) A kind of high performance liquid chromatography system including high pressure pump, sampler, analytical column and SPR detector was build. Using this system, a chromatography of mixed solution of sucrose and galactose was got.