Research On Electric-control Biochip Based On Linear-arrayed Electrode

Electric-control biochip based on linear-arrayed electrode(LEEC Biochip),as one kind of jiTAS(Micro Total Analysis Systems),can control electrically microfluid and biochemical molecules and, is a integrative platform for biochemical analysis, which is integrated multiple functions such as injection, separation, detection and automatic operation etc.Anew method of electric-control for operating microfluid and biochemical molecules accurately has been represented in many recent scientific researches. Arrayed electrodes integrated on microchip, as a good method to generate electric field, have been used widely. And so, we developed a LEEC biochip and a control system for it.My dissertation includes theoretical design of linear-arrayed electrodes and, the procedure of microfabricating microfluidic channel by using an elastomeric materials -PDMS. As a result, different type of PDMS-glass LEEC chip was designed and produced. The LEEC Biochip can be connected with PCB (printed circuit board), thus it can generate a moving electric field by changing time, scope and field intensity discretionarily under single chip processor's control. Meanwhile it is probable to reduce driving voltage and decrease temperature greatly, and so increase resolution of DNA separation.The control kernel of the system is microprocessor c8051F020 and, 54 parallel ports for connecting high voltage system have been expanded. A new network circuit for I/O control was designed to control 54 Pt electrodes to switch discretionarily in 3 states ?high voltage, grounding and suspending. The new system has the characteristic of high integration, low power consumption and high expansibility and, is a good example of extending large numbers of parallel ports to control high voltage apparatus flexibly.We have been succeeded in a primary experiment of separating ds-DNA Ladder 100 using the new LEEC biochip. The experiment demonstrated that the new LEEC biochip can separate DNA fragments successfully and, the whole control system worked normally because of its good compatibility and timing controllability.