Study On A Real-Time Fluorescent PCR Chip System And Its Biological Applications

Biochip technology is a new technology, which developed at the end of the 20th century. Biochip is in the small area, using of micro-processing technology, combined with the chemical synthesis technology to create micro-devices, which have the function of molecular biology test. Analysis and interpretation the information of biological chips will build a bridge between the structure and function of DNA, and push forward the rapid development of life sciences.In this paper, we introduce a novel real-time fluorescent polymerase chain reaction (PCR) chip, which integrated the pretreatment of biological sample, the PCR reaction and the real-time fluorescent detection. We have built a dam in the chamber of the chip, and formed a crack underneath it to separate the white blood cell from the whole blood. Due to its small cavity in the body for PCR reaction, the sample and the use of reagents can be reduced substantially.We have produced a micro-heater and temperature sensor, using micro-chip processing technology. As the biochip is combined with silicon and glass by anodic bonding, there is a very good thermal conductivity effect. And no use of larger heat capacity of the external heater parts, so as to enhance the heating and cooling rate of the PCR reaction, reducing The total time of the PCR reaction. Heater and temperature sensor integrated, allow us to miniaturize the platform of the chip temperature controling, and fluorescence detecting.We have designed the corresponding examination system in view of this chip, which is composed of syringe pump module, temperature control module and fluorescence sampling module.We also used the constructed chip for the examination of HLA-B27 related to ankylosing spondylitis and the results show that the chip is available for the accurate and rapid analysis of HLA-B27 in the whole blood.