Effect Of Mechanical Wounding On Glucosinolates In Arabidopsis Thaliana

The concentration and composition of plant secondary compounds are well known to confer plant resistance to exogenous stress. Glucosinolates (GSs) are a major group of nitrogen-and sulfur-containing secondary metabolites of Arabidopsis thaliana. The rosette leaves of A. thaliana were wounded by cutting approximately 40% leaf area across the apical lamina with scissors, including entire mechanical wounding and half mechanical wounding. The leaves and roots were harvested after the treatment. GSs were extracted from rosette leaves and roots. The dominant GSs were analyzed by HPLC.(1)13 GSs were detected in both rosette leaves and roots including 9 aliphatic GSs and 4 indole GSs. The content of each GS waried in the two organs.4MSOB took the most content in both rosette leaves and roots, but in the indole GSs part, I3M was the highest in the rosette leaves and 1MTI3M was the highest in the roots.(2) Wounding by scissors tend to change GSs contents in both rosette leaves and roots. Totally at 3 h after treatment, GSs contents made the biggest change. The content of GSs in the wounded leaves of wounded plant increased, and increased more in the plant faced to entire mechanical wounding; The content of most GSs in the non-wounded leaves of wounded plant reduced; The content of most GSs in the roots induced after treatment and more insensitive to half mechanical wounding.(3) After treatment, the proportion of each GS in the total GSs changed a lot and also made the biggest change at 3 h after treatment. After entire mechanical wounding, in the rosette leaves, only some high-content GSs like 4MSOB and I3M increasd, the proportion of most kinds of GSs decreased, in the roots, the proportion of aliphatic GSs decreased and the proportion of indole GSs increased; After half mechanical wounding, in the rosette leaves, the proportion of aliphatic GSs in the wounded leaves increased and the proportion of indole GSs decreased, the proportion of aliphatic GSs in the non-wounded leaves decreased and the proportion of indole GSs increased, in the roots, the proportion of aliphatic GSs in the total GSs increased and the proportion of indole GSs decreased.