Effect Of Salt Stress On Glucosinolate Metabolism In Arabidopsis Thaliana

Glucosinolates are nitrogen-and sulphur-containing secondary metabolites in Brassicaceae family. Systematic analysis the glucosinolate content and variation of myrosinase activity with the salt stress at different developmental stages of Arabidopsis in-depth study and provide the initial value in model plant Arabidopsis thaliana.(1) The developmental three periods of A. thaliana with salt stress, we detected the major 11 glucosinolates, including seven kinds of aliphatic glucosinolates which are 30HP,3MSOP, 4MSOB,5MSOP,6MSOH,8MS00,8MT0 and four kinds of indole glucosinolates which are I3M,1MTI3M,4MTI3M, and 4OHI3M.(2) Compared to the control, A. thaliana reflect a certain degree of salt stress after treatment with different concentrations of salt. After treatment of the concentration of 50 mmol/L NaCl, some young leaves of A. thaliana have begun to yellow, for the concentration of 200 mmol/L NaCl, most of the chlorotic leaves, and began to wilt. With the increase of salt concentration, water content of plant tissue appears reduce and cell membrane permeability appear rise.(3) The Glucosinolate content and the proportion of glucosinolate to the total amount of glucosinolate in roots, leaves and petioles of A. thaliana changed after different concentration treatments of NaCl. All components in the concentration of glucosinolates in the root of A. thaliana seedlings root were increased after 200 mmol/L, which the concentration was significantly higher than 150 mmol/L NaCl treatment. Rosette leaves and petioles of aliphatic glucosinolates, indole glucosinolates content group 150 mmol/L NaCl treatment reached the highest levels, significantly higher than the control level.The total amount of glucosinolates, aliphatic and indole glucosinolate in the root of A. thaliana stocklings root appear increase too, in addition to the components outside 3MSOP and 4OHI3M, in 100 mmol/L NaCl was increased after treatment were significantly higher than control level. Leaves and petioles of the total glucosinolates and aliphatic glucosinolates content appear a more consistent trend; the components of glucosinolates response to salt stress were significantly different.The root, stem and leaf glucosinolate content increased, significantly higher than the control level after the high salt concentration (150,200 mmol/L NaCl) treatment in bolting A. thaliana. After five NaCl concentration of salt treatment, the reproductive organs of A. thaliana flowers, pods family aliphatic and indole glucosinolate content were significantly increased.(4) The activity of myrosinase changed after the salt stress, but not obvious in the overall trends. There are no significant relativity between myrosinase's activity and glucosinolate content.