Glucosinolate Accumulation Response To Exogenous Salicylic Acid In Arabidopsis Thaliana

Glucosinolates are a group of nitrogen-and sulfur-containing plant natural products found mainly in the the Brassicaceae family. The predominant biological activities of glucosinolates are associated with their breakdown into various products by the action of the myrosinase, and those products play an important role in plants defend themselves against herbivore and pathogen attack. They have also attracted a lot of interest as cancer-preventive components of the human diet. To study the effect of SA on glucosinolates accumulation, application of salicylic acid (SA) in lanolin to Arabidopsis thaliana rosette leaves was used and glucosinolates pattern was analysis in this study.Application of lanolin alone to Arabidopsis thaliana rosette leaves repress the accumulation of aliphatic glucosinolates. The content of 3MSOP,4OHB,5MSOP and 6MSOH was decreased by 61%,58%,44% and 70% respectively at 48h after treatment and kept in this level afterwards. Whereas the content of 4MSOB and 8MSOO increase slightly at 24h and begin to reduce gradually after 48h. The accumulation of indole glucosinolates I3M was also repressed, whereas lanolin shows no impact on accumulation of 4MTI3M. The total glucosinolate was reduce about 40% at 48,72 and 120h after treatment. At the same time, we find decrease of 3MSOP,4OHB,5MSOP,6MSOH and I3M after treatment and unchanged 4MSOB and 8MSOO in untreated leaves. The total content of glucosinolates in untreated leaves reached the lowest point at 48h and back to normal level at 120h.The negative effect of lanolin to accumulation of glucosinolates can be relieved by SA on some level. The content of 3MSOP,4OHB,5MSOP,6MSOH and I3M are all increase in SA (in lanolin) treated leaves at 120h and the total glucosinolates increase about 30% compare with lanolin treated leaves. At the same time we find that application of SA (in lanolin) alone to Arabidopsis thaliana rosette leaves will affect the glucosinolates pattern of leaves that beside treated leaves in the same plant. The accumulation of 4MSOB and 8MSOO were promoted after exogenous SA treatment and increase 46% and 52% respectively at 48h after treatment. The content of I3M and total glucosinolates almost unchanged in leaves beside SA (in lanolin) treated leaves.