Study On The Development Of CCD Biochip Detector

Biochip technology integrates many new technologies including life sciences, chemistry, microelectronics, computer technology, life informatics, etc. Thanks to the coming forth of biochip technology, it is possible to analyze the functions of gene efficiently and widely, to provide all the presentations of gene in different cases. Moreover, it can address the challenges the traditional biology has faced with. A great importance and research enthusiasms have been paid to it by the research and industrial departments due to its advantages of convenient detection and large volume of information as well as great potential application. It may be a significant revolution of science and technology after LSI. CCD biochip detector is flourishing in the world due to it's advantages of powerful function, efficient detection and easy operation. But, it is nearly a blank in our country. It is important to develop new CCD biochip detecting systems with good performance, reasonable price, independent intellectual property and potential competitive market so as to lay a industrial foundation with good social and economical benefits. A practical design of CCD microarray detector is presented on the basis of combining with microelectronics lithography technology after analyzing the principles and features of two microarray detector namely Laser Confocal Biochip Scanner and CCD Biochip Detector. In the design, a building block Fly-eye lens is utilized to realize uniform illumination so as to breakthrough uniform illumination difficulties of multiple-wave excited source. A high numerical aperture lens is applied to collect fluorescence signal. A logical narrow band filter is employed to eliminate the effects of excited source and scattering light when CCD detects the emitting fluorescence and minimize cross coupling of excited source and emitted fluorescence. A scheme of 12-bit zero-order cooling technology is selected to improve sensitivity and dynamic range for image processing, image smoothing, noise eliminating and image sharpening algorithms are applied in order to improve signal noise ratio. Through automatic position of signal spot and automatic background noise identification,accurate image analyses is obtained.The experiment shows that the developed CCD biochip detector fully reaches the design specifications which means it is great potential applications.