Purpose:Use D-gal injection method to construct the rat aging status,give the aging status of medicine for invigorating spleen in rats of dietetic lavage nursing measure,the success in the construction of the evaluation of aging model and comparative analysis from two aspects of antioxidant and apoptosis herbal medicinal food index influence of aging rats and its related mechanism.Material and method:Selection of SPF level three months healthy Sprague-Dawley?SD?rats 30,They were randomly divided into three groups that the blank,the model and the medicinal food group,Each group of ten.The blank give a 0.9%brine with abdominal subcutaneous is 125mg/kg.d-1.The Model and the medicinal food group give 7.5%D-gal(125mg/kg.d-1)with abdominal subcutaneous,the process is a total of six weeks.After the success of building the experimental intervention,The medicinal food group give medicine for invigorating spleen(10ml/kg.d-1),the blank group and the model group give rice water intervention such as volume,the experimental intervention administered for four weeks.After the last time to fill the stomach twenty four hours,abdominal aorta and then quickly stripped rectus,sliced made with HE staining and microscopic observation of cell morphology change,with ELISA technique in the detection of serum SOD,MDA concentration and the concentration of GSH-PX in the rectus muscle.Inspection with PCR technology stocks rectus muscle of Caspase-3 mRNA and Caspase-8 mRNA expression.Results:1.Inject D-gal after,the model group rats compare with the blank group rats,The growth has slowed,and easily fall back the yellow,dry hair and lethargy,slow response,decrease food intake,without forming mainland.And the medicine for invigorating spleen group rats compare with the model group,found that the behavioral indicators improved,weight growth trend,back hair appear light,good mental state,increase food intake,mainland gradually restored.2.HE staining is the blank group muscle cells in rats skeletal muscle biopsy of fiber thin strip and the nucleus intact.The modle group muscle cells in rats skeletal muscle biopsy of fiber oval or polygonal shape,translocation and atrophy of the nucleus and curing,cells change thin.3.According to the results of ELISA,Concentrations of rectus SOD and GSH-PX in the model group lower concentration than the blank group?P<0.01?and MDA in rectus concentration higher than the blank group?P<0.01?;The medicine for invigorating spleen group concentration of rectus SOD higher than the model group?P<0.05?,and rectus GSH-PX concentration higher than the model group?P<0.01?,and the concentration of rectus MDA lower than the model group?P<0.05?.4.PCR result show that in terms of cell apoptosis,the model group compare with the blank group,the model group rectus Caspase-3 mRNA,Caspase-8 mRNA expression higher than the blank group?P<0.01?,the medicine for invigorating spleen group compare with the model group,the medicine for invigorating spleen group rectus Caspase-3 mRNA,Caspase-8 mRNA expression quantity lower than the model group?P<0.01?.Conclusion:1.To the blank group rats with a large of D-gal can simulate the aging time state,and its simulating aging state consistent with the human body's natural aging.2.Medicine for invigorating spleen can make the aging condition of rats of eliminating free radical enzyme SOD and GSH-PX activity increased,free radicals produced by pests MDA decreased.3.Medicine for invigorating spleen make key protease Csapase-3 and Caspase-8 energy reduced.In some extent hindered the process of apoptosis,and possibly of Caspase pathway is mainly guided by death receptors. |