Correlation Study Between PDCD1 Gene Polymorphisms And Psoriasis Vulgaris With Different TCM Syndromes

Objective: To introduce genetic analysis into the study of TCM syndromes of psoriasis to explore the polymorphism of programmed death receptor 1 gene(PDCD1)in Chinese adults with psoriasis vulgaris with "blood heat and wind dryness syndrome" and "blood deficiency and wind and dryness syndrome".To study the correlation between the clinical phenotype and PDCD1 genotype in patients with psoriasis vulgaris.Specifically,to discuss the correlation between PDCD1 and different TCM syndromes of psoriasis at the gene level,so as to improve the genetic mechanism of current clinical diagnosis and treatment of psoriasis and provide new research ideas for the diagnosis and treatment of psoriasis.Method: A total of 92 patients with psoriasis and 92 normal people in the control group was collected.Their genotypes were identified by PDCD1 gene sequencing.Patients were grouped according to genotypes.The clinical phenotype differences of patients with different genotypes were statistically analyzed by case-control study.Results: 1.This study is the first to study the correlation between PDCD1 gene polymorphism and psoriasis of different TCM syndromes.2.6 SNP sites of immune-related gene PDCD1 were detected in this study,which were PD1.1 Rs36084323,PD1.3 Rs11568821,PD1.4 Rs6705653,PD1.5 Rs2227981,PD1.6 Rs10204525,PD1.9 Rs2227982.3.Stratification analysis of PD1.6 Rs10204525 genotype showed that,with AA genotype as reference,carrying PD1.6 Rs10204525 GA genotype could increase the risk of psoriasis vulgaris in the following groups: age > 40,PASI score >12 and patients without family history of psoriasis.4.Through the study on the correlation between PDCD1 gene polymorphism and psoriasis,it was found that the risk of psoriasis in the individuals with blood heat wind dryness syndrome was 2.17 times higher than that in the individuals with blood deficiency wind dryness syndrome in traditional Chinese medicine.OR =2.17,95% CI =1.12-4.25.Conclusions: 1.PDCD1 gene polymorphism is associated with the risk of psoriasis vulgaris in China.Carrying PD1.6 GA genotype risk of psoriasis vulgaris by 2.27 times.2.Carrying PD 1.6 Rs10204525 GA genotype can increase the risk of psoriasis vulgaris in the following groups: age > 40,PASI score >12 and no family history of psoriasis patients.3.The presence of G allele in PDCD1.6 increases the risk of psoriasis.The PD1.6 Rs10204525 GA genotype is a potential disease-causing genotype,and patients with PD1.6 Rs10204525 GA heterozygote are 2.27 times more likely to cause psoriasis than those with AA homozygote.4.Genotype analysis of PD1.6 Rs10204525 shows that the onset age > 40,PASI score >12,plaque type of clinical manifestations,and psoriasis patients of no family history,PD1.6 Rs10204525 GA genotype is significantly correlated with psoriasis.5.Through the study on the correlation between PDCD1 gene polymorphism and psoriasis,it is found that the correlation between psoriasis risk of patients with blood heat and wind dryness syndrome is 2.17 times higher than that of patients with blood deficiency and wind dryness syndrome.6.The incidence of psoriasis is related to the gene polymorphism of PDCD1 gene,which supports the immune theory and genetic theory of psoriasis.In clinical practice,PDCD1 polymorphisms can be detected to indicate the clinical phenotype and prognosis of patients.