Chaipu Decoction In A Mouse Model Of Liver Depression Asthma Effects Of MAL,CADM1,Il-33,TSLP

Objectives:To explore the effect of Chaipu Decoction on MAL,CADM1,IL-33,TSLP in mouse model of liver stagnatio depression asthma.Methods: A mouse model of liver stagnation type asthma was established by ovalbumin(OVA)sensitization and challenge,and chronic unforeseen stimulation intervention.Thirty 6-week-old female Balb / c mice(body weight 20 � 2g)were randomly divided into 5 groups(n = 6)according to body weight: normal group(group A),asthma group(group B)),liver depression type asthma group(group C,hereinafter referred to as liver stagnation group),Chai Pu Tang + liver depression type asthma group(group D,hereinafter referred to as Chai Pu Tang group),dexamethasone + liver depression type asthma group(group E,hereinafter referred to as dexamethasone group).Rats were adaptively fed for 1 week after purchase.The first stage:Models of liver depression was established on days 1-28,and chronic unpredictable stimulation was performed in the C-E group(fasting for 48 hours,no water for 48 hours,restraint,moist litter,day and night inversion,noise,ice swimming,etc).Group A and B did not do anything.After the modeling was completed,the body weight,sugar consumption test,and forced swimming immobility time of the mice in groups A and C were measured to evaluate liver depression model.The second stage:Group B-E was intraperitoneally injected with 10?gOVA + 400?gAL(OH)3 + 0.2ml saline on day 0 and 7,and groupA was intraperitoneally injected with 0.2ml saline.The B-E group was began to placed in a self-made closed organic small box on the 21 st day,and 1% OVA was atomized with an ultrasonic atomizer,and atomized once a day for 30 min each time,continuous atomization for 7days.0.3ml Chaipu Decoction(1.5g/kg)was administered to the stomach half an hour before each atomization in group D,0.3ml(1mg/kg)of dexamethasone was administered to intraperitoneal injection half an hour before each atomization in group E.Group A was given nebulized with saline.Twenty-four hours after the last atomization,mouse lung tissues were taken for HE staining,and the expressions of CADM1,MAL,IL-33,and TSLP proteins were detected by WB,and the mRNA expression level of the above factors were detected by PCR.Results:1.Identification of liver stagnation model: I.weight comparison: the weight gain of the liver stagnation group was significantly less than the weight gain of ghe contrlo group after the modeling was completed,and the difference was statistially significant(P<0.05).II.Less srgar and water consumption than the control group(due to statistisal analysis of mice due to less sugar and water consumption in each group).III.Forced swimming immobility time: After the modeling,the liver stagnation group took longer than the control group,the difference was statistically significant(P<0.05).2.HE staining showed no significant airway inflammatory changes in the lung tissue of normal mice,regular lumen structure,no thickening of the walls,mice with asthma and liver stagnation had irregular and narrow bronchi lumen and thickened walls.A large number of epithelial cells were fell off,and a large number of inflammatory cells and mecus secretion were seen in the bronchi.bronchal pathological changes in the Chaipu decoction group and the dexamethasone group were alleviated compared with the asthma group and the liver depression group.3.Immunohistochemical results showed that the expression of CADM1 and MAL protein in asthma group and liver stagnation were significantly reduced compared with the normal group(P <0.05),the expression of CADM1 and MAL in Chaipu decoction and dexamethasone group were significantly increased Compared with the liver depression group(P <0.05).The expression of IL-33 and TSLP proteins were significantly increased in the asthma group and liver depression group compared with the normal group(P <0.05),the expression of IL-33 and TSLP were significantly reduced in the Chaipu decoction and dexamethasone groups compared with the liver depression group(P <0.05).4.Real-time quantitative PCR showed that the relative expression levels of CADM1 and MAL mRNA in the asthma group and liver stagnation asthma group were significantly lower than those in the normal group(P <0.05),in addition,the mRNA expression levels of MAL and CADM1 in the liver depression group were lower than those in the asthma group(P<0.05).In the Chaipu decoction and dexamethasone groups,the relative expressions of CADM1 and MAL mRNA were sifnificantly higher than those in the group of liver depression(P <0.05),moreover,the up-regulation of MAL and CADM1 mRNA in chaipu decoction group was more significant than that in dexamethasone group(P<0.05).The relative expressions of IL-33 and TSLP mRNA in the asthma group and liver depression group were significantly higher than those in the normal group(P <0.05),moreover,the increase of TSLP mRNA was more significant in the liver depression group(P<0.05).In the Chaipu decoction and dexamethasone groups,the relative expression levels of IL-33 and TSLP were significantly lower than those in the liver depression group(P <0.05),the expression levels of IL-33 and TSLPmRNA in chaipu tang group were lower than those in dexamethasone group(P<0.05).Conclusion:1.MAL,CADM1,IL-33,and TSLP are involved in the pathogenesis of liver stagnation asthma.2.Chaipu Decoction can regulate the expression of MAL,CADM1,IL-33 and TSLP in asthmatic mice with liver depression,Alleviate the airway inflammation of liver depression asthma,and relieve the condition of asthma.