| Objective:1.To evaluate the clinical efficacy of Zheng's laryngology insufflating medicinal powder No.2 in the treatment of acute pharyngitis,to promote the clinical application of Zheng's laryngology insufflating medicinal powder No.2 and provide clinical data for the inheritance of Zheng's laryngology insufflating medicinal powder No.2.2.To observe the effect of Zheng's laryngology insufflating medicinal powder No.2 on acute pharyngitis rats and to explore its possible mechanism.Methods:1.Clinical trials60 patients with acute pharyngitis were collected from February 2019 to November2019 in Anhui Hospital of Traditional Chinese Medicine.The patients were randomly divided into two groups: the experimental group and the control group,30 cases in each group.The patients in experimental group were treated by Zheng's laryngology insufflating medicinal powder No.2,while the patients in control group were treated Kai Hou Jian spray,and the two groups were set for 5 days as a course of treatment.The data of sex,age and course of disease were recorded.After treatment,the total score of symptoms and signs were compared,and the curative effect of the two groups was observed.2.Animal experiment60 healthy SD rats were randomly divided into 6 groups: blank group,modeling group,model group and Zheng's laryngology insufflating medicinal powder No.2 group.The acute pharyngitis rat model was made by spraying 15% ammonia into the rat pharynx.The high,middle and low doses of Zheng's laryngology insufflating medicinal powder No.2 were 72 mg /(kg · d),36 mg /(kg · d)and 18 mg /(kg · d)respectively.The pharynx was administrated 4 times a day for 5 days.After treatment,the blood of abdominal aorta and pharyngeal mucosa were collected.The contents of C5 a,TXA2 and IL-6 in serum were detected by enzyme-linked immunosorbent assay(ELISA),and the pathological changes of pharyngeal mucosa were observed by hematoxylin eosin(HE)staining.Results:1.Clinical trials(1)After treatment,the scores of symptoms and signs of the two groups were compared:the experimental group was better than the control group in the aspects of pharyngeal pain,pharyngeal hyperemia,pharyngeal swelling,fever,pharyngeal dryness and fever,headache and cough,P < 0.05,the difference was statistically significant.(2)Clinical efficacy comparison: the total effective rate of the experimental group was97%,the total effective rate of the control group was 93%,the two groups were significant and the effect was equivalent,P > 0.05,the difference was not statistically significant.2.Animal experiment(1)The effect of Zheng's throat blowing medicine No.2 on serum C5 a,IL-6 and TXA2 in rats with acute pharyngitis:Compared with the blank group,the concentrations of C5 a,IL-6 and TXA2 in the model group were significantly higher(P < 0.01).Compared with the model group,the high,middle and low dose groups can significantly reduce the concentration of C5a(P < 0.01),the high dose group has a significant advantage,P < 0.01.Compared with the model group,the high,middle and low dose groups can reduce the IL-6 concentration(P < 0.05),the high and middle dose groups have significant effect on the decrease of IL-6 concentration(P < 0.01),the high dose group is significantly better than the middle dose group(P < 0.01).Compared with the model group,the TXA2 concentration in the high,middle and low dose groups decreased significantly(P < 0.01),the high dose group had advantages(P <0.05).(2)The effect of Zheng's throat blowing medicine No.2 on the pharyngeal mucosa of acute pharyngitis:In the model group,a large number of inflammatory cells infiltrated into the lamina propria of pharyngeal mucosa,and the blood vessels were congested seriously.Compared with the model group,the infiltration of inflammatory cells and the congestion and edema of the lamina propria in the pharyngeal mucosa of rats in the high,middle and low dose groups were significantly improved,especially in the high dose group.Conclusions:1.Zheng's laryngology insufflating medicinal powder No.2 has a considerable effect on the treatment of acute pharyngitis,which is equivalent to the control group.2.The mechanism of Zheng's laryngology insufflating medicinal powder No.2 in the treatment of acute pharyngitis may be related to the decrease of TXA2,C5 a and IL-6levels in serum and the promotion of pharyngeal mucosa repair. |
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