Pharmacodynamic Study On The Yinqiao Mabo Powder For Acute Viral Pharyngitis

BackgroundsAcute pharyngitis is a common clinical disease,about 40?60%of which is caused by virus.Yinqiao Mabo Powder is from "Item Differentiation of Warm Febrile Diseases",can clearing heat,with the effect of detoxicating and relieving sore throat.Clinical studies show that Yinqiao Mabo Powder is quite effective for upper respiratory infection,acute laryngitis,and hand,foot and mouth disease,etc,forsythia,honeysuckle,Fructus Arctii and Belamcanda chinensis have been reported to exhibit antiviral activity,so Yinqiao Mabo Powder has potential value for developing new drugs for acute viral pharyngitis.No pharmacodynamics study on Yinqiao Mabo Powder for acute viral pharyngitis has been reported yet.ObjectivesTo develop quality control research on Yinqiao Mabo Powder.To study the antiviral activity of Yinqiao Mabo Powder on Adenovirus,Coxsackie virus and influenza virus in vitro,and on influenza virus in vivo.To study the relieving sore throat,anti-inflammatory,analgesic and antipyretic effect of Yinqiao Mabo Powder.Methods1 Quality control research on Yinqiao Mabo Powder1.1 Quality detection of medicinal materialsDetermine the content of Phillyrin,forsythiaside A,chlorogenic acid,galuteolin,Arctiin and irisflorentin in forsythia,honeysuckle,Fructus Arctii and Belamcanda chinensis by HPLC based on the method of Chinese Pharmacopoeia(2015 version).1.2 Simultaneous determination of five effective components in Yinqiao Mabo Powder by HPLCEstablishing a medthod to determine chlorogenic acid,forsythiaside A,Arctiin,Phillyrin and Irisflorentin simultaneously in Yinqiao Mabo Powder by HPLC2 Study on antiviral effect of Yinqiao Mabo Powder in vitro2.1 Study the antiviral effect of Yinqiao Mabo Powder on HAdV3,CVB3 and H1N1 in vitro.2.2 Study on antiviral effect on H1N1 of Yinqiao Mabo Powder in vivoWe establish the animal model infected by H1N1 in mice by nasal drip to study the antiviral effect on H1N1 of Yinqiao Mabo Powder in vivo.3.Study on relieving sore throat,anti-inflammatory,analgesic and antipyretic effect of Yinqiao Mabo Powder3.1 Study on the curative effect of Yinqiao Mabo Powder on acute pharyngitis model ratsThe ammonia water was used to establish the animal model of acute pharyngitis,the curative effect of Yinqiao Mabo Powder on acute pharyngitis model rats were observed3.2 Study on anti-inflammatory effect of Yinqiao Mabo PowderMouse ear edema model and paw swelling model were used to detect anti-inflammatory effect.3.3 Study on analgesic effect of Yinqiao Mabo PowderAcetic acid writhing and formaldehyde-induced pain model were used to observe the analgesic effect.3.4 Study on antipyretic effect of Yinqiao Mabo PowderFever model induced by LPS was employed to test the antipyretic effect.Results1.Quality control research on Yinqiao Mabo Powder1.1 Quality detection of medicinal materialsThe content of Phillyrin,forsythiaside A,chlorogenic acid,galuteolin,Arctiin and irisflorentin was 0.320%,0.508%,2.880%,0.056%,6.110%,0.210%,the results accorded with the standards of Chinese Pharmacopoeia.1.2 Simultaneous determination of five effective components in Yinqiao Mabo Powder by HPLCThe mothed to simultaneously determine five effective components in Yinqiao Mabo Powder by HPLC was established.2 Study on antiviral effect of Yinqiao Mabo Powder in vitro and in vivo2.1 Yinqiao Mabo Powder could dose dependently reduced the infection rate of the 3 viruses.2.2 The high-dose Yinqiao Mabo Powder could significantly improve the survival time of mice infected by HiNi.Yinqiao Mabo Powder could significantly decrease lung index in mice and the medium-dose and high-dose Yinqiao Mabo Powder could reduce the pathological damage of lung in mice.3.Study on relieving sore throat,anti-inflammatory,analgesic and antipyretic effect of Yinqiao Mabo Powder3.1 Study on the curative effect of Yinqiao Mabo Powder on acute pharyngitis model ratsThe medium-dose and high-dose Yinqiao Mabo Powder could improve the general state of rats,relieved inflammation of pharyngeal mucosa,and had a significant effect on routine blood test and cytokines(TNF-a,IL-6)of rats.3.2 Study on anti-inflammatory effect of Yinqiao Mabo PowderThe medium-dose and high-dose Yinqiao Mabo Powder could reduce the ear and foot swelling degree in mice.3.3 Study on analgesic effect of Yinqiao Mabo PowderThe medium-dose and high-dose Yinqiao Mabo Powder could significantly reduce the number of mice' writhing by acetic acid-induced;all doses could significantly reduce the licking time of mice by formalin-induced in second phase(15?30 min).3.4 Study on antipyretic effect of Yinqiao Mabo PowderAll doses of Yinqiao Mabo Powder could significantly reduce fever temperature caused by LPS.Conclusion1.Quality control research on Yinqiao Mabo PowderThe content of Phillyrin,forsythiaside A,chlorogenic acid,galuteolin,Arctiin and irisflorentin in forsythia,honeysuckle,Fructus Arctii and Belamcanda chinensis accorded with the standards of Chinese Pharmacopoeia(2015 version).The method to simultaneously determine five effective components in Yinqiao Mabo Powder by HPLC is simple,accurate and stable,can provide a reference for the development of Yinqiao Mabo Powder.2.Study on antiviral effect of Yinqiao Mabo Powder in vitroYinqiao Mabo Powder has different degrees of inhibition on HAdV3,CVB3 and H1N1;Yinqiao Mabo Powder has protective effects on death and lung injury in mice infected by H1N1.3.Study on relieving sore throat,anti-inflammatory,analgesic and antipyretic effect of Yinqiao Mabo PowderThe experiment results showed that Yinqiao Mabo Powder has good relieving sore throat and anti-inflammatory effect,which can provide experimental basis for clinical treatment of pharyngeal inflammation;and it has outstanding analgesic and antipyretic effects.