Correlation Between Different TCM Syndrome Types Of Inflammatory Factors And Metabolomics In Community Acquired Pneumonia

Objective:The expression levels of inflammatory factors and metabolomics in peripheral blood of patients with community-acquired pneumonia and TCM syndromes,To observe the changes and differences in inflammatory factors and metabolomics of different syndromes of TCM in community-acquired pneumonia.Provide an objective basis for the substantive study of TCM syndrome types of community acquired pneumonia.Methods:Two TCM syndromes:community-acquired pneumonia(wind-warm lung fever),heat-sucking lung syndrome and wind-heat-sucking lung syndrome were selected,which were divided into the wind-heat-sucking lung group(Cl group)and the heat-sucking lung group(C2 group).Twenty patients were included in the study.20 healthy adults were selected as healthy controls(C3 group).General data,medical history,and physical signs were collected to detect peripheral blood inflammatory factors and plasma metabolomics.Differences in inflammatory factors were analyzed and summarized by SPSS 22.0 software using different statistical methods for different TCM syndrome types of pneumonia patients.Metabolomics used liquid chromatograph + mass spectrometer to simultaneously analyze endogenous differential metabolites in the plasma of C1,C2,and C3 groups,and used database analysis to explore differential metabolites.Results:(1)There was no significant difference in age and gender between patients and healthy controls.(2)There was no significant difference in peripheral blood WBC(*109/L)between C1 group(7.05±2.77)and C2 group(6.83±2.48)(P>0.05);There was no significant difference in CRP(mg/L)between C1 group(23.28±15.29)and C2 group(20.96±15.18)(P>0.05);There was no significant difference in PCT(ng/mL)between C1 group(0.12±0.09)and C2 group(0.12±0.10)(P>0.05);D-dimer(ug/L)was statistically significant(P<0.05)between C1 group(0.38±0.25)and C2 group(0.95±0.80).D-dimer was hot.Patients with pulmonary syndrome have higher blood levels.(3)TNF-?(pg/mL)was statistically significant between CAP patients with C1 group(112.99±52.27),C2 group(166.75±52.27)and C3 group(75.55±14.38).<0.05),compared with healthy people,TNF-? is higher in the blood of CAP patients,and the blood content of patients with TNF-? sputum fever syndrome is higher than that of patients with wind-heat syndrome.IL-6(pg/mL)was statistically significant between patients with C1 group(15.52±6.68),C2 group(20.40±5.04),and C3 group(8.59±3.87).<0.05),compared with healthy people,IL-6 has higher blood content in CAP patients.Compared with patients with wind-heat-sex lung syndrome,IL-6 has higher blood content in patients with hot and cold lung syndrome.MCP-1(pg/mL)in patients with C1 group[182.20(124.73,235.65)],C2 group[241.00(185.68,392.00)]and C3 group[249.70(215.48,310.65)]There was no significant difference in the difference between MCP-1 and CAP patients(P<0.05).There was no significant difference between the syndrome of phlegm-heat and phlegm-heat syndrome(P>0.05),MCP-1 is higher in blood of patients with CAP.SP-A(pg/mL)was compared between CAP patients with C1 group(5422.26±187.98),C2 group(5511.70±183.47)and healthy people(5104.51±498.09).The difference between CAP patients and healthy people was statistically significant(P<0.05).There was no significant difference between SP-A in the syndrome of phlegm-heat and phlegm-heat syndrome(P>0.05).SP-A was CAP patients have higher blood levels.SP-D(pg/mL)was compared between C1 group(3120.45±2741.86),C2 group(3359.35±2827.34)and C3 group(1380.91±810.60).The difference between CAP patients and healthy people was statistically significant(P<0.05).There was no significant difference between SP-D in the syndrome of phlegm-heat and phlegm-heat syndrome(P>0.05).CAP patients have higher blood levels.(4)Differential metabolites between pneumonia patients and healthy people:Dihydrotestosterone,dehydrocorticosteroid,dihydrofolate,serine,histidine,proline,methyldopa.(5)Differential metabolites in patients with pneumonia,phlegm and blood stasis syndrome:Phosphatidylethanolamine,phosphatidylglycerol,N-acetylneuraminic acid,Acylcamitine.Conclusion:1.The levels of TNF-a,IL-6,MCP-1,SP-A and SP-D in peripheral blood of patients with CAP are higher than those of healthy people.2.Differences in D-dimer,TNF-?,and IL-6 in peripheral blood of patients with CAP who have wind-heat-sucking lung syndrome and phlegm-heat-sickness syndrome.3.There are differences in hormone and protein metabolism between CAP patients and healthy people.4.CAP patients with wind-heat attack and lung syndrome Pulmonary syndrome differs in lipid metabolism.