Analysis Of The Curative Effect Of Ornipural On Acute Renal Failure In Canine

Objective:In order to provide reference for pet clinical medication,artificial making canine acute renal failure and using ornipural drug to cure dogs ARF.the clinical effect of ornipural on acute renal failure in dogs was observed and Ornipural effect on ARF treatment was analysised from heamatology and histopathology.Methods:Sixteen healthy dogs(5±0.5 kg)were randomly divided into 3 groups,namely experimental group 1,experimental group 2 and control group.The canine ARF was produced by using a 50%glycerol(10 mL/kg)muscle injection combination with gentamicin(120 mg/kg)muscle injection.When ARF was successfully produced,the patients were treated according to the control group and the experimental group respectively,and the urine volume was measured daily.Starting treatment begin on the day of ARF clinical symptoms appear,control group to adjust the salt water and electrolyte balance,infection controand so on,this are regular support therapy method in the treatment of ARF;on the basis of routine support treatment,experimental group1 was treated with intravenous drip of Ornipural(5ml/kg),while 2 groups were treated only with ornipural(5ml/kg)intravenous drip.Blood was collected from the jugular vein on The day of clinical symptoms,3 days after treatment and 7 days after treatment,blood routine indexes were measured,including total white blood cell count(WBC),lymphocyte(LYM),neutrophile granulocyte(GRAN)and monocytes(MONO);Renal functional biochemical indicators were measured,including urea nitrogen(BUN),creatinine(CREA)and blood phosphorus(PHOS);Determination of potassium in electrolyte index(K~+).The kidney of the experimental group and the control group were respectively taken after treatment that fixed by pressure perfusion with formalin at 100 mmHg for 24 h.After fixation,these were imbedded in paraffin and stained with haematoxylin-eosin,the pathological changes of the kidney after treatment were observed in the experimental group and the control group.Results:Results were as follows:1.The production of the canine ARF:Dogs were in the process of making 5 day clinical symptom such as vomiting,no urine,spirit was depressed,blood test,blood urea nitrogen(BUN),creatinine(CREA),blood(PHOS)phosphoric acid root,were higher than in the normal range,that ARFto make success.None of the sixteen dogs were killed in the process.2.Treatment situationIn the control group,ARF was died in the second day of treatment;In the experimental group 1,one of the dog with ARF died on the second day of treatment,two died on the 3rd day of treatment,and one died on the 5th day of treatment,and one was cured after 7 days of treatment;In the experimental group 2,Four of the ARF dogs died on the first day of treatment,and one died the following day.3.Changes in urine volumeAfter treatment,the urine was much better than that in the control group,and the urine volume of experimental group 1 of dogs was close to the average daily urine volume of the normal dog on the seven days of treatment.Compared with the control group,the urine volume of the first day dog was 9.60±14.05ml,and the amount of urine was significantly increased in experimental group 1 after treatment four day and five day(P<0.05),which were 54.00ml and 44.00ml;Compared with the control group’one day,the urine of experimental group 1 on after treatment six day and seven day was extremely decreased(P<0.01).4.Changes in blood routine parametersAfter seven days of treatment,the surviving dogs’figures of the WBC,GRAN,MONO and LYM had all returned to normal range in the experimental group 1,which were 10.50×10~9/L、8.30×10~9/L、0.5×10~9/L、1.7×10~9/L respectively.The figure of WBC on the 7th day in the experimental group 1 was significantly lower than that before the treatment in experimental group 2 and the control group,and the figures respectively were 10.50×10~9/L,19.27±5.45/L and 19.54±5.72/L,which were significant differences(P<0.05).5.Changes in renal function indicatorsThe figures of the BUN,CREA,PHOS and K~+which were dogs’renal function indexes gradually decreased after treatment in experimental group 1.In addition,the surviving dogs’figures of CREA、PHOS、K~+had returned to normal range on the 7th day,the figures were 1.30mg/dL,6.70mg/dL and 4.00mmol/L respectively.Nevertheless the figure of BUN was slightly higher than the normal range,which was31.00mg/dL.Compared with all of sick dogs’renal function indexes before treatment,the figure of BUN significantly decreased(P<0.05)on 3rd day which was 50.25±25.09mg/dL,and 4 indexes also significantly decreased(P<0.05)in experimental group 1.6.Pathological changes of kidney tissuesThe renal tubular epithelial cells of the control group and the experimental group2 had different degrees of necrosis,swelling and detachment under the microscope.From the clinical symptoms of kidney biopsy figure is mainly manifested as glomerular and balloon adherent each other,The substrate increase,interstitial edema,a large number of inflammatory cells exudation,small part of the proximal convoluted structure disappeared and renal tubular epithelial cells cavitation,these are the pathological features of ARF.In the control group and in the experimental group,the renal tubular cavity of the two groups of dead dogs had proteinuria,and various fragments were in the lumen;After 7 days of treatment,the renal tubules in the kidney section of the experimental group 1 were significantly repaired,and there were no urinary catheter and cell debris,and the glomerulus was not adherent to the balloon.Conclusions:Ornipural can reduce the number of blood CREA,BUN and PHOS,and it can reduce the number of inflammatory cells in the blood.On the basis of conventional support therapy,it is better to use ornipural therapy dog ARF,which can prolong the survival time of the dog.