Establishment Of Renal Failure Model And Liver And Kindey Injury Induced By Ethylene Glycol In Canine

Objective:To establish a canine model with renal failure induced by ethylene glycol and to explore the change law of biochemical indexes and renal ultrasonography results in different stages of canine glycol injury,so as to provide practical scientific basis for the accurate clinical diagnosis of animal renal failure.Method:In this experiment,8 healthy adult canines at the age of 1-2 years old were selected,each weighing 4-10kg.The 8 canines were evenly divided into 2 groups,the acute renal failure group and the chronic renal failure group(n=4),with 4 canines in either group.The canines in the acute renal failure group were fed with ethylene glycol solution at a dose of 1.5 mL/kg per day.During the treatment,the daily clinical symptoms were observed,and B-ultrasonography together with blood biochemical examinations were performed before the toxin challenge and every 2 days thereafter.The canines in the chronic renal failure group were fed with ethylene glycol solution at a dose of 0.75 mL/kg per day.Also,the daily clinical symptoms were observed,and B-ultrasonography as well as blood biochemical examinations were performed before the toxin challenge,with additional tests every 2 days and every 7 days after the challenge,respectively.The main items of the blood biochemical examinations include the determination of creatinine(CREA),urea nitrogen(UREA),Phosphorus(Phos),Calcium(CA),alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALKP)and glutamyl transpeptidase(GGT).After natural death,the pathological sections were made from the liver and kidney tissues and checked by microscopy.Results1.Blood biochemical index variations of canines in the development of renal failure induced by ethylene glycolThe changes of CREA,UREA,Phos,CA:When symptoms like mental sluggishness and decrease or even loss of appetite occurred,the value of CREA(173±8.49),UREA(9.75±0.49),Phos(3.69±1.12),CA(1.87±0.26)deviated significantly from the normal range(44?159,2.5?9.6,0.81?2.19,1.98?3.00),indicating damage in kidney cells.2.B-ultrasonography result variations of canines in the development of renal failure induced by ethylene glycol:when poisoned by ethylene glycol,the echo of the kidney was considerably enhanced in contrast to that before the challenge experiment,even higher than the spleen echo,leading to abnormal images in the B-Us.3.The pathological section examination results of canines with renal failure induced by ethylene glycol:As for the renal tissue from the acute renal failure group,abscission,necrosis,blisters and fat deformation were discovered in the epithelium of renal tubular,and crystals were found inside the renal tubular;Also,dilation and congestion were detected in glomerular;For the chronic renal failure group,connective tissue proliferation and vascular congestion occurred in renal tubulointerstitial area as well as the liver region.4.TheinfluenceofliverinjurybyethyleneglycolThe changes of ALT,AST,ALKP and GGT:The indexes of ALKP are in the normal range.While the value of ALT(159±13.95),AST(56±25.25),GGT(8±3.31)were higher than the normal value(10?100,0?50,0?7),when mental sluggishness and decrease or even loss of appetite appeared,which shows a certain damage of liver cells in the late stage of ethylene glycol poisoning.As for the high dose ethylene glycol group,steatosis and vascular congestion appeared in the liver tissue and spleen tissue,hemorrhage was reported.For the low dose ethylene glycol group,tissue proliferation and vascular congestionoccurredintheliverregion.Conclusions:The canine model with acute/chronic renal failure induced by ethylene glycol was established and verified by the results of blood biochemical indexes,B-ultrasonography and pathological section examinations.And through accumulating blood biochemical data and B-Us images of ethylene glycol poisoning,this study provides substantial evidence for clinical applications on animals.