The Experimental And Clinical Research Of Compound Angelica Tincture In Treating Vitiligo

Objective:Vitiligo is a common depigmentation skin disease, and the treatment is varied. The aim of this study was to ensure the therapeutic effect and efficacy of compound angelica tincture (CAT) in treatment of vitiligo through animal experiment and clinical observation.Method:1. Animal experiments:the experiment was carried on from October 2014 to January 2015. In the experiment, ninety C57BL mice were divided into 6 groups:normal control group, vitiligo model group,0.1% Methoxsalen group, low concentrations of CAT group, medium concentrations of CAT group and high concentrations of CAT group. Firstly, we established a mouse model of vitiligo with H202, and gave different treatment to each group; secondly we detected the content of serum Monoamine oxidase (MAO) Cholinesterase (ChE) and plasma Tyrosinase (TYR) in each group, and took the images in dermoscopy and analysed images with softwares and also collected the other indice such as average optical density (0D), pigment-containing follicle count and repigment area ratio.then finding out the difference of each group in histopathology. Finally, using spss19.0 to analyze the data of each group.2. Clinical observations:The participants were patients who visited the dermatological outpatient department of Wuhan General Hospital of Guangzhou Military Command during March and October 2014. After selected follow to enroll standard,31 voluntary participants were adopted. According to Traditional Chinese Medicine(TCM) syndrome differentiation, the vitiligo was divided into 5 types:deficiency of liver and kidney type, rheumatoid accumulated heat type, congestion in channels type, deficiency of spleen and stomach type and stagnation of the liver type. All of participants were treated with compound angelica tincture for 3 month, then comparing the degree of repigmentation of 5 types, and finally the result contrast with animal experiments and draw conclusions.Results:1. High concentrations of CAT can significantly increase the content of ChE and TYR in Vitiligo mice, and significantly reduce the content of MAO in Vitiligo mice. Especially TYR and MAO can return to normal level (p>0.05).medium concentration of CAT can increase the content of ChE and TYR in Vitiligo mice.Reducing the content of MAO in Vitiligo mice, but the restoring levels is lower than high concentration (p<0.05).Low concentration of CAT can slightly reduce the content of MAO in vitiligo mice and had no significant effect on content of ChE and TYR. High concentrations of CAT can increase the number of coloring hair follicles in vitiliginous lesions of mice, and melanin density also increased significantly, even reaching normal levels (p>0.05). medium concentration of CAT can increase the melanin density and the number of coloring hair follicles. The difference of repig-mentation between medium concentration and high concentration of CAT was statistically significant (p<0.05). Low concentration of CAT slightly increased the number of coloring hair follicles in mice and had less effect on repigmentation2. Clinical observations show that the CAT was more effective in liver and kidney deficiency type and liver stagnation type the repigmentation rate is 58.9% and 53.3% respectively, while poor efficacy in the congestion in channels type which repigmentation rate was 34.1%. The difference was remarkable. (p<0.05)3. Use the high concentration of CAT to treat of vitiligo mice and patients, repigmention rate was 57.1% and 52.6% respectively. Conclusion:CAT can increase the content of ChE and TYR in Vitiligo mice can reduce the content of MAO. CAT can increase the number of coloring hair follicles in vitiliginous lesions of mice.CAT is safe and effective for treatment of vitiligo.It is worth recommending in clinic.