| Objective: In this study, through the establishment of SD rats with acute closed soft tissue contusion model, using the late renowned medical expert martial Zheng Huai Xian old professor of clinical experience Zheng’s Zhu Gui-San as a therapeutic drug, basic medical insurance and Sichuan industrial injury insurance drug list drugs Zhunzi Z11020464 Qili San(Beijing Tong Ren Tang Tong Ren Tang Pharmaceutical Co., Ltd.) for comparison, by internal medicine treatment, recovery was observed in rats injured limb; and to detect NO content and its morphological observe, explore Zheng’s Zhu Gui-San on blood circulation after acute soft tissue, anti-inflammatory, analgesic effect, and clinical treatment for side acute soft tissue injury to provide a theoretical reference.Methods: Two 75-month-old SD rats, modeling after 72 rats were weighed and recorded, and numbered rats were randomly divided into three groups(n = 72), namely experimental group(Zheng’s Zhu Gui-San), drug control group(Qili San), the control group. And then randomly divided into four time points group(1,3,7,10, n = 6). Experimental group, drug group, the control group were given preoperative Zheng’s Zhu Gui-San, Qili San saline gavage twice daily. Drawn at each observation point relative weight of rats weighed accordingly; evaluation of soft tissue injury symptom index; light microscope morphology and rate performance; NO content was measured nitrate reduction; using statistical software(SPSS16.0) on processing and analyzing relevant data.Results:(1) Weight Results: The first day of Zheng’s Zhu Gui-San group, Qili San group, the control group showed no significant difference in body weight(P> 0.05); the third day of surgery Zheng’s Zhu Gui-San and blank have more significant difference between the control group(P <0.05); the seventh day surgery Zheng’s Zhu Gui-San and control group differences(P <0.05), there were significant differences(P <0.05 Zheng Gui scattered and Qili San surgery group); there were significant differences(P <0.05) the tenth day of surgery Zheng Gui scattered group and control group, there was a significant difference(P <0.05) Zheng’s Zhu Gui-San and Qili San San.(2) Soft Tissue Injury Index: soft tissue injury Symptomatology index showed a continued downward trend. After modeling the first day, the third day score between the groups rather, there was no significant difference(P> 0.05) by the test; the 7th day, Zheng’s Zhu Gui-San group, Qili San scores were lower than the control group group, Zheng’s Zhu Gui-San group, Qili San group and control group were tested there was a significant difference(P <0.05); on the 10 th, Zheng’s Zhu Gui-San group and Qili San loose group and control group were There was a significant difference(P <0.05), whereas no significant difference(P between Zheng’s Zhu Gui-San group and scattered groups Qili San> 0.05);(3) Histological observation: Over time, each group organization edema, inflammatory cell infiltration reduction, showing a trend to reduce the inflammatory response, Zheng’s Zhu Gui-San group, Qili San group than the control group; Microscopic data score results: The first three days, Zheng’s Zhu Gui-Sanscattered group and the control group there was a significant difference(P <0.05), Qili San group and control group, there was a significant difference(P <0.05); the first seven days, Qili San surgery group and Zheng Gui powder group were compared with the control group, there were significant differences(P <0.05); the first 10 days, Qili San surgery group and Zheng Gui scattered groups were significantly with the control group differences(P <0.05);(4) NO content(μmol / gprot): comparison between groups: The first day, no Qili San surgery group and Zheng Gui scattered groups was significant(> 0.01), Zheng’s Zhu Gui-San group and Qili San casual group with the control group, and there were significant differences(P <0.05); third, Zheng’s Zhu Gui-San group and scattered group Qili San significant difference(P <0.05), Zheng Gui San surgery group and the control group there was a significant difference(P <0.05); the seventh day, Zheng’s Zhu Gui-San group and Qili San casual group compared with the control group, there were significant differences(P <0.05), no significant difference in comparison(P Zheng’s Zhu Gui-San group scattered group and Qili San> 0.05); the tenth day, Zheng’s Zhu Gui-San group scattered, Qili San group compared with the control group, there were significant differences(P <0.05); in the group show: Zheng’s Zhu Gui-San scattered groups of four time points were There was no significant difference(P> 0.05); Qili San group, the third and the seventh day there was a significant difference(P <0.05); blank control group at each time point was no significant difference(P <0.05);Conclusion: Zheng’s Zhu Gui-San scattered local tissue can promote the repair of soft tissue injury. Zheng Gui San surgery can improve soft tissue injury in acute symptoms, signs, can effectively inhibit the overproduction of NO, to avoid excessive damage to the soft tissue and promote repair of soft tissue. |
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