Effect Of Zheng Guishen Powder On PGE2 And Histomorphology After Acute Soft Tissue Injury In Rats

Objective: The hospital used a traumatology surgery Zheng’s Zhugui-San, in Professor Zheng Huaixian book "Chinese medicine orthopedic experience" clearly states that with blood, swelling, analgesic effect, treating muscle damage(especially on the new injury best effect). By observing and recording technique Zhugui-San on acute soft tissue injury in rats symptoms, histology, prostaglandin E2(PGE2) changes, and with the treatment of bruises, blood stasis bleeding common side Qili San Acute the effect of soft tissue injury do comparison, explore San Zhugui-San acute soft tissue injury treatment effect. Provide a reference for the system of research and clinical applications Professor Zheng Huaixian traumatology prescription system.Methods: Two 75-month-old male SD rats, weighing 253.26 ± 14.27 g, adaptive feeding into the laboratory for 7 days, all rats were left thigh using hair removal in the popliteal fossa 1 cm combat modeling. Selected 72 successful model rats were randomly labeled with a marker, then weighed records, check randomly divided into Group A: saline group as a control group, group B: Qili San group the control group, Group C: Zhugui-San scattered as the experimental group. Drug after modeling the experimental group and the control group will be made by gavage turbid suspensions orally administered normal saline control group, were free feeding water. When the degree of swelling of the skin at each point of observation with a local modeling area, congestion petechiae, limb movement disorder, on the first day of 9:00 1,3,7,10 take tissue samples, sliced, light microscope, evaluate the organization changes in morphology. Produced by changes in local tissue concentrations of PGE2 enzyme-linked immunosorbent assay. And the results are analyzed using the software for data processing.Results:(1) Weight: The rats in the expe riment, the control group showed a slow upward trend in weight, 7 days, 10 days were statistically significant compared with the preoperative Zhugui-San group(P <0.05); control group, body weight gradually reduced after m odeling, 7 days began to rise, the first seven days, day 10 and Zhugui-San group than in the scattered statistically significant; Zhugui-San group of patients weight gain is obvious, in making On day 7, day 10 after molding, with the blank control group were statistically significant compared.(2) Symptomatology Index: three groups of rats symptom index from the beginning of the modeling show a downward trend in the first day, the control group, the control group, no patients after modeling Zhugui-San set of mutually significant difference(P > 0.05), no statistically significant; the first three days, the control group and the control group was statistically significant after modeling, but compared with the preoperative Zhugui-San groups was not statistically significant; the first 7 days after modeling, blank group and the control group was highly statistically significant, the control group patients with Zhugui-San groups was statistically significant; the first 10 days after modeling, blank group and the control group, patients Zhugui-San groups, were statistically highly significant(P <0.01); 4 time points in the control group patients with Zhugui-San groups were not statistically significant.(3) PGE2 indicators: changes in PGE2 concentration in the control group of rats increased the previous three days, after the fall, while the control group and the group scattered Zhugui-San has shown a downward trend in the first 7 days after modeling technique Zhugui-San group concentration and the concentration of the control group was statistically significant, but the concentration of Qili San group compared with blank group was not statistically significant.(4) histological score: three groups of rats histological scores have declined in the control group and Zhugui-San group than in the control group scattered fall faster. The first 3 days, 7 days, 10 days Zhugui-San control group compared with the control group were statistically significant, while Zhugui-San group and the control group at all time points than no significant significance.Conclusion: congestion arising after bruising Zhugui-San can improve the acute injury in rats scattered, swelling subside, and reduce local tissue injury in PGE2 content relieve pain, relieve claudication, can be seen scattered Zhugui-San for acute soft tissue injury from effective action to reduce swelling and pain.