The Optimal Concentration Of Intra-articular Injection Of Medical Ozone In Dog’s Knee Of Early Osteoarthritis

Objective: Through copying the dog knee osteoarthritis model,|ocomparison of the optimal concentration about the three kinds of lowconcentration of medical ozone intra-articular injection for the treatment on kneeosteoarthritis.Methods:To select40healthy male dogs randomly divided into4groupsof A (air group) and B (10ug/ml medical ozone group), C (20ug/ml medicalozone group), group D (30ug/ml medical ozone group)，each group of10, Onthe letf knee joint for N group (normal control group). By Hulth method used tocreate OA model for each group of domestic dogs’ right leg knee joint, Atfer thesuccess of the building, A group with air injection on the right side of the kneejoint cavity. B，C，D group of injection of different concentration of medicalozone1times a week, each time5ml, a total of4times.Put to death the expeirmental dogs atfer treatment, line on near the medialfemoral condyle cartilage tissue pathology observation and Mankin rating； Takejoint fluid with nitrate reduction method to detect the contents of TNF alpha.Select building respectively, when building a successful and put to death beforeto measure and compare the kennel knee joint mobility and X-ray.Results: In the treatment of8w group A atfer knee joint mobility atferserious limitations Atfer the treatment of B，C, D group irght knee degrees was increased when modeling a successful, But C group knee degrees increasedsignificantly than B, D group (P <0.05); Four groups articular cartilagepathological damage was found in the right knee, group A was most seirous, thelightest cartilage lesions in group C; Mankin lowest score in group C, comparedwith the N group had no statistical significance (P>0.05); With A, B, Dcompared different concentrations of ozone group was statistically significant (P<0.05); Ozone treatment group joint fluid TNF alpha content in group C wasstatistically significant than A, B，D group significantly decreased (P <0.05).Conclusions:20ug/ml group of ozone can reduce arthritic cartilage injury,enhance the body’s ability to eliminate rfee radicals and can treatment ofdegenerative arthritis,30ug/ml ozone group because of its high degree ofoxidation make organization cell lipid peroxidation, led to the destruction ofarticular cartilage tissue structure.