The Effect Of Intra-articular Injection Of Medical Ozone In Rabbits Knee Of Osteoarthritis

Objective: Observed the impact of articular injection of medical ozone knee osteoarthritis in rabbits and discuss the treatment of different concentrations of medical ozone on osteoarthritis.Methods:32 rabbits were randomly divided into 4 groups of A (air group), B (25μg/ml medical ozone group), C (40μg / ml medical ozone group), D (80μg / ml medical ozone group), each right knee joint were modeled, then give the knee articular injection of 2 ml medical ozone once per week for four weeks. 3 days after the last injection the rabbits were killed, taken the cartilage near the medial femoral condyle for pathological observation and Mankin score; take joint fluid to detect NO concentration; measure the degree of knee joint at the time before modeling, modeling successful and before sacrifice.Results:1. When modeling successful each group right knee degrees is lower than before modeling.Before sacrificed B, C, D group right knee degrees is higher than when modeling successful.Before sacrificed B group right knee degrees is significantly higher than C group and D group (P<0.05);2. Articular cartilage pathology damage was found in right knee of each group, articular cartilage of B group was mild degeneration, Mankin score of right kee articular cartilage was no significant difference than in the left knee (P> 0.05);The articular cartilage was significantly degenerated in C group and D group, Mankin scores of their right kee articular cartilage were significant difference than in the left knee (P<0.05);3. NO concentration of the joint fluid of right knee rise in each group, the NO concentration of B group reduce the maximum degree in relative to C group and D group.Conclusion:Three levels of medical ozone were able to reduce the inflammation of osteoarthritis.40μg/ml and 80μg/ml of medical ozone can cause structural damage to articular cartilage,and the damage was increased with increasing concentrations of medical ozone.