| Allium Victorialis L. is the Liliaceae Allium herbs, Its warm, spicy, non-toxic side effects,Chinese medicine can be used for chill cold and oue fullness disease treatment. There are records of ancient medical books,the effect of Allium Victorialis L.is hemostatic, stasis, pain and phlegm. In recent years, one after another at home and abroad has been reported in a variety of medicinal value. For the utilization of Allium Victorialis L. provide strong evidence. Jilin wild Allium Victorialis L. has excellent growth environment, green natural and pollution-free,Congxiang bodied taste, its rich contents have significant medicinal value, It is an ideal functional vegetables. In this paper, Allium Victorialis L. as raw materials, combined with ultrasonic assisted ethanol extraction of the active ingredient in Allium Victorialis L.flavonoids and saponins extracted, and were optimized by response surface methodology respective extraction process, With crude flavonoids extract(flavonoid content of 23.18%),crude saponin extract(saponin content of 28.42%), and two components 1: 1 mixture of quality for the repair of the experimental group of mice and liver injury caused by alcohol non-specific immune suppression in mice to study immune regulation, Explore the effects of different functional components, Liver damage and immune suppression itself is an expression of the inflammatory response,This paper aims to find out the name of the Allium Victorialis L.in the liver, improve the function of the immune system component, indirect explanation Allium Victorialis L. anti-inflammatory effect, in order to provide a basis for the development and utilization of Allium Victorialis L.Test results are as follows:1.Ultrasonic assisted method of active ingredients Allium Victorialis L.flavonoids optimization experiments, crude flavonoids extraction rate evaluation, test results show: When the solid-liquid ratio was 1: 61.74, 96.17% ethanol concentration, ultrasonic time was 1.54 h, the extraction rate of crude flavonoids reaches the maximum is 0.5870 percent.2.In ultrasound-assisted extraction method for extracting active ingredients Allium Victorialis L.saponin optimization experiments to extract crude saponin yield index, the results showed that When the solid-liquid ratio was 1: 40.33, 75.48% ethanol concentration, ultrasonic time of 1.56 hour, the extraction rate of crude saponins reaches a maximum of 1.6337 percent.3. Repair of liver injury in mice With test crude extracts of flavonoids, saponins crude extract, and a mixture of two functional components of the experimental group caused by alcohol, the liver tissue of mice MDA, TG, GSH-PX content measured and to calculate the liverindex. Experimental results show that flavonoids crude extract, crude saponin extract and the mixture of the two components can reduce the MDA in liver tissue, TG content, increase the content of GSH-PX, reduce liver index, the role of good protection of the liver, wherein flavonoids and saponins mixed group performance better.4. In order to test the role of immunocompromised flavonoids crude extract, crude saponin extract, and a mixture of two functional components for the experimental group of mice caused by cyclophosphamide, the mice liver tissue acid phosphatase, serum acid phosphatase, serum lysozyme correction carbon clearance index and index test organ index, the results show:Flavonoids crude extract, crude saponin extract and the mixture of the two components can increase the content of liver tissue acid phosphatase, serum acid phosphatase, serum lysozyme correction carbon clearance index also improved, spleen index, thymus index and liver index three indicators uptrend, showing good immunomodulatory effect, the effect of which flavonoids and saponins mixed group better. |
PDF Full Text Request