Scanning The Single Nucleotide Polymorphisms (SNPs) Of Omentin Gene In The Southern Chinese Han Population With Type2Diabetes Mellitus, And A Correlation Analysis Of The Polymor Phisms And Hypertriglyceridemic Waist

Objective Scanning omentin gene promoter regulatory region, thesignal peptide region and the entire exon in Southern Chinese Han Populationwith type2diabetes mellitus. Identification of omentin gene single nucleotidepolymorphisms (SNPs), to explore the correlation between its polymorphismsand hypertriglyceridemic waistMethods100patients in the study were from the Southern Chinese HanPopulation with newly diagnosed type2diabetic mellitus. According tohypertriglyceridemic waist phenotype into two groups of HTGW＋and HTGW－.Omentin gene was amplified by PCR, two-way direct DNA sequencing toidentify the research object of omentin gene SNPs. To analyze the difference ofomentin gene polymorphism genotype frequency，allele frequency distributionand clinical variables in the two groups. All of the data were analyzed bySPSS16.0.Using the logistic regression models to analyze the correlationsbetween omentin gene SNPs and clinical biochemical indexes. Results (1) There are14SNPs spots in omentin gene and12SNPs were firstreported, which are verified by bi-directional sequencing. There are-269A/G、-260A/G、-171G/A、-82A/G、-36A/G and-31G/A in promoter regulatory region.Gly16Gly (A/G) is in the signal peptide region. There are Ser84Ser(C/T)、His86His(C/T)、 Gly97Gly(C/T)、 Ser106Asn(G/A)、 Val109Asp(T/A)、Asp114Asp (C/T) and Tyr312His(T/C) in the entire exon.(2) The significantdifference was found in the distribution frequency of allele T and A of omentingene Val109Asp (T/A) in the two groups of HTGW＋and HTGW－. The rest ofthe polymorphic allele frequency distribution of the difference was notstatistically significant.(3) Compared with AA homozygous mutations, AG+GGgenotype of omentin gene Gly16Gly(A/G) has the higher level of high densitylipoprotein cholesterol in the HTGW＋group; compared with TT and AAhomozygous mutations, CT+CC genotype of omentin gene His86His(C/T) andTA genotype of omentin gene Val109Asp (T/A) have the higher level ofpostprandial b1ood glucose in the HTGW＋group; compared with GGhomozygous mutations, GA genotype of omentin gene Ser106Asn(G/A) has thehigher level of HbA1c, all the difference were statistically significant(P＜0.05).However, omentin gene polymorphisms with all the clinical variables were notstatistically significant (P>0.05) in the HTGW－group.(4) The univariatelogistic regression and stepwise regression analysis results: After removing theage, sex and others clinical variables, FBG、TG and HTGW are independentlyassociated with His86His (C/T), they are also independently associated with Val109Asp (T/A), the former odds ratio (OR) and95%confidence interval (CI)were4.421(1.705-11.465)、2.982(1.098-8.098)、3.256(1.179-8.992); the latterwere3.218(1.236-8.379)、3.381(1.159-9.863)、3.345(1.024-10.931). Thesignificance of this correlation was statistically significant (P <005). Conclusions In the HTGW＋group of type2diabetes，the mutations ofomentin gene Gly16Gly(A/G)、His86His(C/T)、Val109Asp (T/A) andSer106Asn(G/A) are closely related to the high density lipoprotein cholesterol、postprandial b1ood glucose and glycosylated hemoglobin A1c. The mutationsmay be significantly increased in the glucose and lipid metabolism disorder ofdiabetic patients. The mutations of His86His(C/T) and Val109Asp (T/A) areclosely related to hypertriglyceridemic waist. It may be a genetic factor, whichis influences the progression of diabetic vascular complications. It can be usedas a genetic indicator for primary prevention of diabetic vascular complications.