| Objective: To observe the osteoarthritis of knee (KOA）rabbit modles treated byblood-letting puncture therapy and explain the possible mechanism in treating KOAthrough experimental study, providing scientific evidence that can be used to treat KOAclinically.Methods:32New Zealand rabbits were randomly divided into four groups: the blankgroup(group A),the model group(group B),the blood-letting group(group C) and the druggroup(group D),eight rabbits in each group. In group A,the rabbits were fed normallywithout doing other managements.In group B, rabbits were built into models.In groupC,the rabbits were treated by blood-letting therapy after having been moulded successfully,twice per week.In group D,after having been moulded successfully, the rabbits were givenmedication every day,the total treatment lasting for eight weeks. Then, detect theintraosseous pressure value and the volume of IL-lβ and TNF-α in the serum.Results: Compared with group A, the intraosseous pressure of the rabbits in group B ishigher than that in group A. The statistic shows that there exists significant difference（p＜0.01）, which means that the models were built successfully. Compared with group B,theintraosseous pressure of the rabbits in group C and D decreases significantly andsignificant difference was observed （right side p＜0.01). The difference between group Cand D has no statictic meaning（p>0.05）,which means both blood-letting therapy and drugcan decrease intraosseous pressure, with almost the same effect. Compared with group A,the volume of IL-lβ and TNF-αin the serum in group B significantly increase (p <0.01);compared with group B, the volume of IL-lβ in the serum in group C and D significantlydecrease and significant difference is observed （p＜0.01）, while the volume of TNF-αin the serum in group C and D decrease significantly (p <0.01) with significant difference.The difference between group C and D has no statictic meaning（p>0.05）, which meansboth blood-letting therapy and drug can decrease the volume of IL-1β、TNF-α in the serum.Conclusion: The blood-letting puncture therapy can effectively reduce the volume of intraosseous pressure, and the volume of IL-lβ and TNF-α in the serum, which may beone of the mechanism for blood-letting puncture therapy to treat knee osteoarthritis. |
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