Clinical And Experimental Research On Hyperlipidemia Treated With Point Injection With Compound Angelica Injection

Objectives:To observe the clinical curative effect of point injection at“Feng long”(ST40) in the treatment of hyperlipidemia and theinfluence of the treatment on the ATP-binding cassette transporterA1（ABCA1) in the liver tissue of the hyperlipemia-modeled rat inorder to investigate the mechanism of point injection at“Fenglong”on blood lipids，so as to provide a scientific basisfor clinical treatment.Methods:72HLP patients were randomly divided into2groups.30patientswere in the treatment group with point injection.36patients werein the control group with acupuncture. The treatment group weretreated with of point injection at “Feng long”(ST40) every otherday，10times as a course,2courses all together. The control groupwere treated with acupuncture at “Feng long”(ST40) for30minutesevery other day，10times as a course,2courses all together. Bloodsamples were taken to measure the levels Total cholesterol（TC）、Triglyceride（TG）、Low-density lipoprotein cholesterol（LDL-C）、 High-density lipoprotein cholesterol（HDL-C）in the beginning andend of the treatment.The experiment study was also conduted.40healthy SD rats wererandomied into a normal control group, a model group，a pointinjection group, an acupuncture group,10rats in each group. Thenormal group was fed with basal forage, and other3groups were fedwith high fat forage each day to establish hyperlipidemia rat model.After the modeling, the point injection group start point injectiontreatment，the acupuncture group start acupuncture treatment at thesame time. The control group and the model group, the same crawl,but no treatment.At the end of the treatment,the expression of ATPbinding cassette transporter A1(ABCA1) mRNA and protein in livertissue were assayed by in situ hybridization(ISH)、RT-PCR and byWestern blot.Results:1Clinical results1.1Comparison of comprehensive therapeutic effect: The clinicaltherapeutic effects of the treatment group were obviously higherthan that of the control group.There were significant differencesbetween the treatment group and the control group in clinicaltherapeutic effects(P<0.05).1.2Comparison of chinese medicine symptom score:The intra-groupcomparison suggest treatment group indecreasing symptom scorebetter than the control group(P<0.01)。The inter-group comparisonsuggest treatment group indecreasing symptom score better than thecontrol group(P<0.05).1.3Comparison of lipid lowering total effective: The total effectof lipid lowering effective rate in the treatment group was90% ,in the control group was85.7%,.There were no differences betweenthe two groups(P>0.05).1.4Comparison of lipid: The intra-group comparison has significantdefference in lipid target TC，TG,LDL-C lowering,suggest that thetwo groups have therapeutic effect. The inter-group comparisonsuggest the treatment group has better effect in decreasing thelevel of TC than the control group (P<0.05).2Experimental results:2.1Comparition of the lipid: The content of TC,TG and LDL-C wereapparently increased(P<0.01), and the HDL-C were significantlydecreased in the model group(P<0.05); compared with the model group,levels of TC,TG,LDL-C were significantly decreased were increasedin the treatment and control group（P<0.01，P<0.05）; the treatmentgroup has better effect in decreasing the level of TC than thecontrol group (P<0.05).2.2Comparition of the expressin of ABCA1mRNA and protein: Theexpression of ABCA1mRNA and protein were significantly decreasedin the model group (P<0.01); compared with the model group,theexpression of ABCA1mRNA and protein were significantly increasedin the treatment and the control group(P<0.01，P<0.05); comparedwith the control group，the expression of ABCA1mRNA and proteinwere significantly increased in the treatment group(P<0.05).Conclusions:The clinical research results shows that acpoint injection at“Fenglong”has a significant therapeutic effect.Ordinary acupun-cture also have some effect, but the clinical efficacy of acupointinjection was significantly better than ordinary acupuncture.Animal studies show that acupoint injection on Fenglong can significant promote the ABCA1mRNA and prote of the liver tissueof HLP rat so as to infer the transcription and translation of ABCA1,thereby contributing to the RCT, improving abnormal lipid metabo-lism of hyperlipidemia.