| Objective:Hyperlipidemia refers to the abnormality in the body fat metabolism or transporter. A large number of epidemiological surveysthat hyperlipidemia is an important risk factor for atherosclerosis, high blood pressure, coronary artery disease. In recent years, non-drug therapy for hyperlipidemia attention, especially the clinical appl i cat ion of acupuncture in the treatment of hyperlipidemia, has made significant progress, the effects are more significant, showing a unique advantage. The purpose of this experiment is to hyperlipidemia rat model for the study of reverse cholesterol transport pathway as an entry point to observe the rats in each group lipid levels, hemorheology, macrophage inflammatory factor, liver and aortic arch tissue morphology. changes ABCA1, SR-BI, PPARγ, IGF-1gene and protein expression, electro-acupuncture the Fenglong on reverse cholesterol transport pathway the prevention and control of the mechanism of action of hyperlipidemia.Methods:Select40adult male SD rats, after adaptive feeding for1week, the rats were randomly divided into blank control group, model+normal diet group, model+high fat diet EA group, model+normal diet EA group. Each one has ten rats. After the end of treatment, the rats in each group through carotid artery blood, serum lipids (TC, TG, HDL-C, LDL-C) content and blood rheology indicators (high shear blood relative index, whole blood low shear relative index, plasma viscosity, erythrocyte aggregation index). Peritoneal macrophages of rats in each group were then separated and analyzed by flow cytometry plus fluorescently labeled antibody inflammatory cytokines molecules ICAM-1, MCP-1, TNF-α, IL-6levels. HE staining, is aim to observe the tissue morphological changes of the the rats liver tissue and the aortic arch in the light microscope. Detection of liver tissue of ABCA1and SR-BI, PPAR y, IGF-1gene (mRNA) expression and protein content.Results:①The with blank control group relative comparison, the remaining three groups plasma TC values are significant with the rise, there are very significant with difference (P<0.01), model+ordinary feed group and model+high-fat feed electric acupuncture group plasma LDL-C values significantly with the rise, the difference has very significantly with sex (P<0.01), HDL-C values significantly decreased, significant sex (P<0.05) difference; model+ordinary feed group phase comparison model+high-fat feed EA group and model+normal diet EA plasma TC, LDL-C values were significantly reduced, and there was a significant difference (P<0.01).(2) In addition to the model+EA group beginning material remaining rats hemorheology (whole blood high shear relative index of whole blood relative index, plasma viscosity, erythrocyte aggregation index) compared with the blank control group, both an increase in type differences (P<0.05, P<0.01); model+common starting material group Hemorheology model+normal diet EA group was significantly decreased (P<0.01).③In addition to the model+normal diet EA group group, the rest of the rats in each group peritoneal macrophages ICAM-1, MCP-1, TNF-a, IL-6compared with the blank control group were significantly increased (P<0.01, P<0.05). Compared with the model+ordinary feed group model+feed electric needle group in addition of IL-6have decreased significantly (P <0.05), the expression of ICAM-1, MCP-1, of TNF-a were very significantly with the decline, with a significant differences (P <0.01).④model+normal diet group rat liver tissue visible moderate to severe diffuse fatty degeneration of the liver tissue damage and fat deposition. The arterial wall of the aortic arch uneven thickness, intimal thickening and lipid calm, severely damaged aortic endothelial cells, endometrial discontinuous foam cells are deposited in the intima lower spot visible lesions fatty streaks fat. Given acupuncture treatment of hyperlipidemia successful modeling and high fat diet, the ordinary start feeding feeding two sets of circumstances are different degrees of improvement, the severity of liver tissue has been reduced, the structural part of the repair of liver cells, reduce fat deposition, aortic arch organizations vessel wall structure was repaired clear, reduce of subendothelial lipid and inflammatory cell infiltration, membrane stretch film some repair.⑤with blank control group, model+normal diet EA group, and the remaining two groups of ABCA1and SR-BI of PPARγ were significantly lower (P<0.01), and IGF-1significantly increased (P<0.01); model+normal diet group compared with the model+high fat diet EA group only SR-BI and IGF-1two numerical differences (P<0.05), while the model+normal diet EA group ABCA1, SR-BI, PPAR y were significantly (P<0.01) reduction in IGF-1significantly increased (P<0.01); model+high fat diet EA group, model+normal diet EA group ABCA1, SR-BI, PPAR Y, IGF-1were significant differences (P<0.05, P<0.01).Conclusion:EA Fenglong reduce hyperlipidemia rats TC, LDL-C levels, increased HDL-C levels, improve blood concentration in high-fat state, sticky, poly, condensate at high risk status, reduce macrophage inflammatory cytokines of ICAM, MCP-1, TNF-a and IL-6, improve rat liver and aortic arch morphological changes effectively suppress the occurrence of steatosis remove fat cells protect vascular endothelial reduce fatsedimentary, early atherosclerosis vascular wall has a protective effect. Electro-acupuncture the Fenglong by elevate cholesterol reverse transport the path two important functional protein of ABCA1and SR-BI expression, accelerated lipid metabolism, to achieve the purpose of the treatment of hyperlipidemia. |
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