Evaluation On Selection Of Disinfectant And Disinfective Ditermination In Scale Chicken Farm

The epidemic of scale chicken farm is the results of comprehensive factors caused, but the main factor is the invasion and diffusion pathogenic microorganism from external environment or pathogenic microorganism diffusion in chicken themselves. Disinfection potential of five disinfectants (A, B, C, D, E) were determined for screening the disinfectants with strong disinfection potential, and then the efficacy eveluation of disinfection was made to these screened disinfectants. Based on above results, it will provide reference for chicken house to use correctly disinfectants for epidemic prevention and establishing disinfection procedure.The minimum bactericidal concentration(MBC) of five disinfectants to reference strains (Escherichia Coli 8099) and E.coli isolated from chicken house was determined by limiting dilution assay. The result of five disinfectants to antisepticize reference strains was determined by suspension quantitative bactericidal test. Bacteria content in chicken house was determined by natural sinking method, and the effect of five disinfectants were also evaluated objectively through the on-the-spot disinfection of chicken house and disinfecting chicken house with chicken together.The result showed the MBC value of disinfectant E was smallest, but its bactericidal action was strongest, then disinfectant D, and bactericidal action of disinfectan C,B,A were weaker. After mixing 5 disinfectants with E.coli mixture for 1 min,5 min and 10 min, it was found that antimicrobial activity of disinfectant E to E.coli was higher than disinfectant C,B and A significantly (P﹤0.01), but there was significant difference between E and disinfectant D(P﹥0.05). And field disinfection trial in chicken house indicated that the effect of disinfectant E was superior to disinfectant D, and disinfectant E had significant effect to air, wall, ground, crib and waterer as well as cages surface, and disinfectant E influenced obviously mortality of chiken (P﹤0.05).After disinfecting, mortality of trialed chicken decreased 2.86%. So disinfectant E is safe and efficient, and it is fit to disinfect chicken house with chicken together,and it has good potential of application and popularization for disinfecting in livestock farm.