The Detection Of H9Subtype Avian Influenza Virus In The Soil Of Chicken Farm And The Selection Of Chemical Disinfectant

China has the largest quantity of poultry in the world, the soil pollution problem around chicken farms is getting worser with the development of poultry breeding industry, Once the soil has been contaminated, the nature of soil would be changed, not only destroy its normal functions, but also contaminate the groundwater, even pose a threat to human health. The research about protective disinfectant, which can be large scale used to handle contaminated soil is becoming hot. This study selects the H9subtype avian influenza virus as a representative. First, we established a detecting method of H9subtype avian influenza virus in soil, then the related disinfectants have been experimented to control the pollution of the H9subtype avian influenza virus among chicken farms.1. A RT-nested PCR method was established for detection of H9subtype avian influenza virus in soil. The HA gene of H9subtype avian influenza virus was selected as the target gene, two specific pairs of primers were designed, a RT-nested PCR detecting method was developed. This method has good specificity, the results of sensitivity test showed the limit of detection was0.5TCID50/g. By adding this virus in the soil five days later, it still can be detected through the survival time testing. The above results showed this RT-nested PCR detecting method was quick, specific and sensitive.2. By adding glutaraldehyde or chlorine dioxide to the soil, the sterilization effect on the H9subtype avian influenza virus was evaluated. The results showed:Disinfecting effect of two kinds of disinfectants were significant difference compared with the control group (P<0.001). The results of soil carrier test indicated:0.05%glutaraldehyde for10min or300mg/L chlorine dioxide for2h could achieve a100%killing rate. Field experimental results showed that the killing rate was100%by using0.1%glutaraldehyde for48h or300mg/L chlorine dioxide for24h. These results showed glutaraldehyde and chlorine dioxide can effectively control the pollution of the H9subtype avian influenza virus in soil.