Polymer Enzyme Sensor Research

1.The galactose biosensor based on microporous polyacrylonitrileA galactose biosensor is obtained by immobilizing galactose oxidase (GAO) in a microporous polyacrylonitrile (PAN) thin film. The effects of pH, potential and temperature on response current are studied. The optimum pH and apparent activation energy of enzyme-catalyzed reaction are 7.1 and 31.1 kJ mol^-1, respectively. The response current of the biosensor increases linearly with the increasing galactose concentration from 0.02 to 1.60 mmol dm^-3. The Michaelis-Menten constant value (Km) is 12.15 mmol dm^-3. The biosensor shows good operational stability and reproducibility. The galactose biosensor is characterized with cyclic voltammogram, FTIR and UV-Vis.2.A noninterference polypyrrole glucose biosensorA noninterference polypyrrole glucose biosensor was constructed based on the four-electrode cell consisting of a polypyrrole film electrode, a polypyrrole-glucose oxidase electrode, a counter electrode and a reference electrode. Two polypyrrole films were same except one with glucose oxidase (GOD) and another without GOD. The difference between the currents at two working electrodes was measured directly by precision biopotentiostat in the same solution containing substrate. The effects of potential, pH and temperature on the glucose biosensor were studied. The calibration curve was determined under the given circumstance. It was discussed mainly to eliminate interference of ascorbic acid through the four-electrode cell at different potential. The glucose biosensor was characterized with FTIR and UV-Vis.