Spleen Soup Of Depression Model Rats Estrogen Levels And Hippocampal Morphology

Objective :Study the anti-depression effect of Guipi Decoction and the mechanism is approached.Method:(1)40 common female Wistar rats were selected,which having the same weight,behavior points and learning memory ability were divided into the normal group,model group,saline group and Chinese medicine group, 10 rats per group.(2)Except the normal group,rats in other 3 group were established the depression model by received chronic unpredictable stress stimulus. (3)No medicine was given to the normal group. The model group was exerted with lavage action. The saline group was lavaged with 2ml saline 0.5h earlier the stimulus every time. The Chinese medicine group was lavaged with 2ml Guipi Decoction at the same time.(4)The learning memory ability was estimated with Morris water maze video tracking system. (5) Serum estradiol,follide-stimulating hormone and luteinizing hormone were determined by using radioimmunoassay.(6)The histology changes of CA3 neurons were observed with HE staining. Neural number was measured with BI—2000 medical image analysis system.(7)The data was expressed by X|-±S,statistical analyzing with SPSS 11.0.Result:(1)Compared with the normal group,the swimming distances of the model group and saline group increased obviously. But the index of the Chinese group did not change significantly compared with the normal group.(2)Compared with the normal group,serum estradiol concentrations in the model and saline groups decreased obviously. While Chinese medicine group was not significant in statistics. (3) Compared with the normal group,follide-stimulating hormone and luteinizing hormone concentrations in the model and saline groups increased obviously.While Chinese medicine group was not significant in statistics.(4)In the CA3 areas.there were abundant pyramidal cell layers,and cells were complete,compact,well-arranged,and the edge of cells was clear in the normal and Chinese medicine groups. While the layers of pyramidal cell decreased,and the cleft of cell enlarged,and the array of cell was sparse,even many cells were deficient in the model and saline groups. Compared with the normal group,the neuron number of the model and saline groups decreased obviously and the Chinese medicine group did not change significantly.Conclusion :(1)Guipi Decoction might antagonize the decreasings of rat learning memory ability caused by chronic unpredictable stress.(2)Guipi Decoction could maintain the excretion of serum estradiol in the chronic unpredictable stress state.(3)Guipi Decoction could hold the excretion of follide-stimulating hormone and luteinizing hormone in the chronic unpredictable stress state.(4)Guipi Decoction might protect the hippocampal CA3 neurons from the injuries by chronic unpredictable stress.