Significance And Expression Of TGF-β1 And TβRⅡ In Lacrimal Gland Epithelial Tumors

ObjectiveThis study is to investigate the expressions of TGF-β1, TβRⅡand PCNA in lacrimal gland epithelial tumors,it evaluates the possible role of TGF-β1 signaling pathway in the occurrence and development of lacrimal gland tumor. Simultaneously, it clarifies whether TGF-β1 plays role in cell proliferation by analysis the relevance of TGF-β1 and PCNA.That will be benfit to the diagnosis and treatment,and to evaluate the prognosis of lacrimal gland tumor as a theoretical basis.Methods1. Collect the linical and pathological information of patients diagnosed as lacrimal gland epithelial tumors from November 2003 to November 2008 in Tianjin Eye Hospital.2. Immunohistochemical staining was used to detect the expression of TGF-β1, TβRⅡand PCNA in lacrimal gland epithelial tumors.3. The data was analyzed by SPSS 13.0 statistical software.Results1. TGF-β1, TβRⅡprotein were mainly localized in the cytoplasm, and PCNA protein was localized in cell nuclei,their expressions have nothing to do with ages,sexs, tumor size and course of disease.2. The AOD values of TGF-β1 protein respectively were 0.0192±0.0062,0.0857±0.0484,0.2414±0.0751 in the normal group,benign group and malignant goup,significant difference among them(P<0.05); The AOD values of TGF-β1 protein respectively were 0.0626±0.0213,0.2370±0.0778 in no recurrence group of the benign epithelial tumors and recurrence group,significant difference between them(P<0.05). The AOD values of TGF-β1 protein respectively were 0.2390±0.0743,0.2502±0.0713 in no recurrence group of the malignant epithelial tumors and recurrence group,no difference between them(P>0.05).3. The AOD values of TβRⅡprotein respectively were 0.2548±0.0292,0.2498±0.0285,0.2497±0.0314 in the normal group,benign group and malignant goup, there were no significant differences among them (P> 0.05); The AOD values of TβRⅡprotein respectively were 0.2504±0.0301,0.2501±0.0334 in no recurrence group of the benign epithelial tumors and recurrence group,no difference between them (P> 0.05). The AOD values of TβRⅡprotein respectively were 0.2489±0.0279,0.2481±0.0241 in no recurrence group of the malignant epithelial tumors and recurrence group,no difference between them(P>0.05).4. The AOD values of PCNA protein respectively were 0.133±0.0213,0.17±0.0234,0.2445±0.0323 in the normal group,benign group and malignant goup,significant difference among them(P<0.05); The AOD values of PCNA protein respectively were 0.1499±0.0468,0.2431±0.0314 in no recurrence group of the benign epithelial tumors and recurrence group,significant difference between them(P <0.05). The AOD values of PCNA protein respectively were 0.2344±0.0306,0.2647±0.0261 in no recurrence group of the malignant epithelial tumors and recurrence group, significant difference between them(P<0.05).5. The positive correlation was showed between protein expression levels of TGF-β1 and PCNA in lacrimal gland tumors (P<0.05); protein expression levels of TGF-β1 and TβRⅡwere no significant correlation (P> 0.05).Conclusion1. TGF-β1 participates in the occurrence and development of lacrimal gland tumor,and possibily play a important role in reccurence of benign epithelial tumors.2. TPRⅡprotein possibily was no loss in lacrimal gland tumors, and it had no relationship with TPRⅡprotein that TGF-β1 can not inhibit the cell proliferation.3. PCNA can reflect cell proliferation of lacrimal gland tumor, and evaluate the clinical biological behavior of lacrimal gland tumor.4. TGF-β1 can't play a biological role of inhibiting the cell proliferation with the increasing of the degree of tumor malignancy and the high expression of TGF-β1 may promote tumor cell to proliferate.