Clinical Research On Recipe Bushen Huoxue Repair Eutopic Endometrium In The Treatment Of Endometriosis Associated Infertility

BackgroundEndometriosis (EM for short) is a common gynecological diseases, is caused by pelvic pain and infertility in women of childbearing age one of the reasons. Currently the Western endometriosis pathogenesis remain unclear. Although laparoscopy endometriosis as a diagnostic gold standard, but because of its invasive and expensive prices, the medical profession in the search for non-invasive diagnostic method. Treatment at home and abroad have used drugs, surgery and assisted reproduction treatment, however, no single treatment can completely cure endometriosis. Chinese medicine believes that the pathogenesis of endometriosis is infertility kidney and blood stasis. Many clinical and experimental studies have shown that kidney and activating blood circulation can effectively improve the pregnancy rate in patients with EM. The current treatment of Chinese medicine Bushenhuoxue EM Mechanism of infertility has reached the molecular level and found that Chinese medicine can regulate EM BHD patient's immune, endocrine function, repair defects in eutopic endometrium, endometrial receptivity to improve So as to improve the pregnancy rate. However, its mechanism and the role of specific target has not yet been finalized.ObjectivesTo objectively assess the clinical efficacy and enhance the deduction The purpose of this study as a starting point from proteomics technology to explore the side BHD EM infertility patients in the repair defects of the possible mechanisms of eutopic and infertility in the pathogenesis of EM and drug intervention target for the EM is not Pregnant patients with a standardized program of Chinese medicine clinics, kidney Recipe for Chinese medicine infertility treatment in the clinical application of EM and promotion provide an objective scientific basis for the further development of new drugs to provide a theoretical basis.MethodsIn this study, clinical trial methods, patients were selected from May 2010-March 2011 in Guangdong Provincial Hospital outpatient and inpatient gynecology department of EM after infertility and abortion patients 3-6 months later, the use of Chinese medicine EM Bushenhuoxue side intervention in patients with infertility for 3 months for infertile patients with EM before and after treatment mid-secretory eutopic endometrium and induced abortion after 3-6 months later mid-secretory endometrium of women of childbearing age, the use of Fluorescence difference gel electrophoresis (2D-DIGE) tag, separation of proteins, Decyder difference analysis software for analysis, identify the normal control group, EM infertility group, herbal differentially expressed proteins in the intervention group, and using matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF/TOF) corresponding differences in protein peptide mass fingerprinting, and then search the database to identify some different protein spotsConclusionThrough this study, we obtained the following 4 conclusions:1.the establishment of normal women and patients with endometriosis and infertility medicine before and after treatment eutopic endometrium Proteome Cells.2.Found 35 different express proteins by differential software analysis, then identified 24 different proteins using mass spectrometry identification, suggesting that infertility may be associated EM. Chinese medicine treatment in which the protein predicted targets of 14 species.3.28 kinds of different proteins on the bioinformatics analysis, summarized Bushen Huoxue Recipe repair mechanism may be divided into the following five aspects:①regulation of intracellular signal transduction pathways:the membrane with protein (annexin I), protein kinase inhibitor 1γ(YWHAG), protein kinase inhibitory protein 1α/β(YWHAB);②change the composition of the cytoskeleton, increased cell invasion:cytoplasmic actin 2 (ACTG1), vimentin (VIM), micro-Tubulin a 4A chain (TUBA4A),β-actin-like protein 2 (ACTBL2);③regulating endometrial cell secretory protein:apolipoprotein A-1;④reduce the sensitivity of endometrial cells, promote cell over proliferation:anti-proliferative protein (PHB), protein kinase inhibitor 1γ(YWHAG), protein kinase inhibitory protein 1α/β(YWHAB), heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNPA2/B1), heterogeneous nuclear ribonucleoprotein core Protein C1/C2 (hnRNPC1/C2);⑤in free radical production and abnormal expression of clear balance of the enzyme, promoting the formation of inflammatory compounds:membrane with protein (annexin I), superoxide dismutase (SOD2), DJ-1 protein (PARK7), glutathione S-transferase (GSTP1). Bushen Recipe fix the possible role of eutopic defects above four mechanisms involved in target proteins. Predicted a direct interaction of two proteins-protein complexes.In summary, the present study, separation of the joint DIGE-MALDI TOF/ TOF mass spectrometry-proteomics bioinformatics analysis systems, to find normal women with endometriosis and infertility patients before and after treatment Bushen Recipe Eutopic endometrium between proteins. This not only reveals the molecular basis of disease provides a new technical methods, but also for in-depth study of endometriosis-related infertility, the pathogenesis and mechanism of traditional Chinese medicine offers a new therapeutic target information and opportunities.