| Objectives Cardia gland cancer has a high morbidity in our country.Patients with this disease have a very low five years survival rate and the metastasis is very quickly. This disease is harmful to health. In recent years, although surgery technique, chemotherapy and radiotherapy have a so quickly development, the survival rate has not risen parallelly.The reason is microm-etastasis through the lymph or blood vessels before the surgery. Investigation found that some of the carcinoma could restrain the growth of metastatic focus, no metastatic carc-inoma would grow quickly only after the original carcino-mawas ablated. Researchs of these reasons made many bl-oodvessel growth depressor found.Endostatin is one of the-m, which was found by O'Reilly in Harvard 1997. It wasdistilled from Eoma culture medium first. It is a proteoly-tic fragment of collagen type XVⅢ, which is a proten in-habitor of angiogenesis.Its molecular weight is 20KD.Manyacademicians have had many studies on it recently. It is lcocated at tissue fundus, mostly in some of highly bloodvessel organs.Endostatin restrains the fashion of new bloodvessel, including chicken embryo allantoid velum, big rat cornea, artery annulus culture medium,and so on. Endosta-tin can restrain the transference of inner cell and the mul-tiplication of blood vessel endotheliocyte cell stimulated by bFGF and so on at cell levels. Many academicians ca-rried through a lot of studies on the characteristics of en-dostatin. And they proved that endostatin had close relati-ons with many carcinomas'growth,metastasis and prognosis,such as lung cancer, liver cancer,kidney cancer and so on.It can reduce the blood vessel numbers of carcinoma, inc-rease the wither of carcinomacell. So that it can resist carcinoma. Endostatin has been in clinical research in America. We have not found the related study on expression of endostatin in cardia gland cancer by now. We wanted to analyse the influence of endostatin to cardia gland ca-ncer long-dated survival and the relationship between expression of endostatin and clinical and physiological charact-eristics in the cardia gland cancer through examining the levels of endostatin in the tissue of cardia gland cancer, in that of nub and in that of benign cardia disease. More-over, to evaluate the synthesis therapy value in the applic-ation of endostatin to cardia gland cancer. Method: We analysed the endostatin levels of cardia gland cancer and in the nub of cardia gland cancer by applying immunohistochemical staining(SP method). We took the tissue of cardia from the benign cardia diseasepatients in thecorresponding period for comparison. The samples were all from the cardia disease patients therap-yed in the Department of Thoracic Surgery of the Forth Hospital of HeBei Medical University from January 1996 to December 1996, of which 62 cases were cardia gland cancer, 14 were benign cardia disease patients. All cases were not under chemotherapy and radiotherapy. Results: (1)The expression of endostatin was located at the carcinoma cells,the cardia mucosa, the vascular endothelial cells, plasm cells, and part of lymphocyte cytoplasm. (2)Comparison of the expression of endostatin in different cardial tisssues: the total positive expression in cardia gland cancer was 78.12%±5.71%, which was higher than that in the tissues of nub and benign esophageal disease, 49.34%±5.58%and 40.36%±8.56%respectively(p<0.01), and positive expression of endostatin in nub was higher than benign cardial disease(p<0.01). (3)Relationship between the expression of endostatin and clinical and pathophysiological characteristics in cardia gland cancer tissue:the expression of endostatin in cardia gland cancer was closely related to theinvasion degree of primary tumor, clinical TNM grades and cell differentiation of cardia gland cancer (p<0.01), but not to area lymph nodes metastasis, age, sex, habits of eating and history of family (p>0.05). (4)The correlations among the expression of endostatin and clinical and pathophysiological characteristics in the cardia gland cancer:th...
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