Roles Of Long Non-coding RNA In Cardia Cancer Cell Apoptosis

Background: This study aims to find the differentially expressed genes and build coding non-coding network between the expression genes by bioinformatics method in cardia cancer(CA),then verified the selected genes is associated with CA cell apoptosis through the molecular biology experiments.Methods: CA and normal tissue samples were obtained from patients of The First Affiliated Hospital,Henan University of Science and Technology and Anyang Tumor Hospital.The genome-wide analysis of lncRNA(Long non-coding RNA)expression in these tissues was performed using the Human LncRNA Array v2.0(8×60K,Arraystar),we found apoptosis related gene ontology(GO)term through the enrichment analysis,a coding-noncoding gene co-expression(CNC)network was constructed by the Pearson coefficient.Finally,the selected lnc RNA was verified by qRT-PCR and CA cell lines verification.Results: We found that 5139 lnc RNAs were differentially expressed in CA.Among these,3026 lncRNAs were up regulated more than two-fold relative to normal tissues and 2113 lncRNAs were down regulated.GO identified 42 genes which was significantly related to biological processes of "anti-apoptosis"(GO term: 0006916).There were 2 mRNAs and 50 lncRNAs in CNC network,the Pearson correlation coefficient is greater than or equal to 0.97.Then,qRT-PCR data verified 1 downregulated lncRNA(RP11-98F14.10)in CA which was randomly selected according to another 29 samples.Finally,we found that over-expression of this lnc RNA through the lentiviral vector increased CA cell apoptosis in CA cell line(SK-GT-2).Conclusion: Our results demonstrated that there were lots of differentially expressed genes in cardia cancer,and lncRNA(RP11-98F14.10)may be associated with cell apoptosis.Further study of these lncRNAs could provide useful insight into CA biology.