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Comparison Of The Sterilization Ability Of Bacteriostats And The Effect On Seedlings Growth

Posted on:2008-04-14Degree:MasterType:Thesis
Country:ChinaCandidate:X Y LiFull Text:PDF
GTID:2120360218951671Subject:Botany
Abstract/Summary:PDF Full Text Request
The sterilization ability of 39 kinds of bacteriostats were compared. Preliminary control microorganism contamination with those bacteriostats through the study of in-vitro sterilization and the influence on seedlings growth was studied. The results showed that agricultural antibiotics ethylicin, chemical pesticide Junshabaoguo, Sophoraflavscens Ait,medicinal antibiotic Gentamycin Sulfate Injection and disinfectant1229 had obvious bacteriostasis on germs and mildews, and the bacteristatic spectrum were wide. Chemical pesticide Junshabaoguo had both inhibitory effects on spore germination and mycelium growth. The effective sterilization of Junshabaoguo, isinfectant1229 and Gentamycin Sulfate Injection were low and they didn't have influence of MS mother liquid and high pressure sterilizating on their sterilizating ability. But all bacteriostats had some toxicity on the seedlings growth. High concentration of Junshabaoguo inhibited the seedlings from growing up, while low concentration could promote the growth of the plant; Gentamycin Sulfate Injection and disinfectant1229 had obvious restrainable effect on rooting occurring and sprouts forming of seedlings. The plant growing retardant Pix had strong sterilization ability and also had obvious increasing the sterilization ability of bacteriostats. While concentration was 10mg/L, Pix had obvious promoting effect of seedlings growth development and the influence on node was indistinctive. Contamination control showed that bacteriostats could not cure the infectant seedlings after soaking treatment. But it was a better way to add the bacteriostats in the culture media to inhibit contamination. Junshabaoguo, Gentamycin Sulfate Injection and disinfectant1229 could prevent contamination occurring effectively.
Keywords/Search Tags:plant tissue culture, bacteriostat, plant growth development, contamination control
PDF Full Text Request
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