Research Of Micro Environment CO₂ Enrichment Control System For Sugar-free Plant Tissue Culture Based On PLC

It is a good way for plantlets' commercial application in agriculture to develop automated industrial-scale culture facilities by using advanced environmental control technology. But there are a lot of complexed problems about the environmenteal factors effecting the growth and morphasis of plantlets, so it is urgent to develop environmental control system which adapt to use in plant tissue culture . The object of this research is to develop an environmental control system which can directly monitor and control the micro-environment in which plantlets were planted during the whole period of the culture;seek the way to putrify the air in culture box;study the effects of CO2 enrichment on culture plantletoWe designed an environmental control system for sugar-free plant tissue based on PLC. In this paper introduced the study of fuzzy controller which was used for CO2 supplying control from CO2source-high-pressure steel tin to culture box, studied the control of relative humidity and CO2concentration in the culture box and effects of CO2 enrichment (enrichment concentration was 900-1100mol mol-1) on the growth of chrysanthemum plantlet and tried to clean the air in the culture box by cyclingThe results indicated that this environment control system operates normally and reliably .It can keep the CO2 concentration and relative humidity in setting scope; it is good to the growth of the culture plantlets to clean the air in culture box by cycling; CO2 enrichment can promote the growth and photosynthetic and can increase the percentage of shooting The number of new leaves of the chrysanthemum plantlet cultured under CO2-enriched condition is 1.85 times of the plantlet cultured under CO2-nonenriched condition; The number of the roots of the chrysanthemum plantlet cultured under CO2-enriched condition 5.67 times greater compared with the plantlet cultured under CO2 nonenriched condition. The sum lengths of the roots of chrysanthemum plantlet cultured under CO2-enriched condition were 10.6 times as great as the plantlets cultured under CO2-nonenriched condition.