Studies On Some Aspects About Medium Of Plant Tissue Culture

Based on the tissue culture system establishment of more and more plant species,the technique of tissue culture is used widely in many fields of plant science.In the process of establishing tissue culture system of a particular plant species,many aspects should be studied,of which,the medium is the most important one.In this paper,14 kind basal mediums were compared and analyzed using multianalysis methods.The chemical stability in different storage conditions of NAA,IBA and KT solution,and the absorption effect of active charcoal for IAA,KT and 2,4-D were studied.The main results are as follows:1.Fourteen kind basal mediums used in plant tissue culture research commonly,were studied using the methods of cluster analysis and principal components analysis.The results showed that:(1) Under the 1.40 classification level according to Chi-square distance,all the 14 kinds basal mediums could be classified into 4 groups.The first group include 3 kinds basal mediums(White,Miller,Heller),the second group include 6 kinds basal mediums (MS,LS,ER,NT,NN,H),the third group include 3 of them(B₅,N₆,SH),and the fourth group include 2 ones(DKW,WPM);(2) For the macro-elements,the first and the second principal components represent Na₂SO₄ and KNO₃,and there weight coefficient are -0.38256 and -0.49446 respectively,and for the micro-elements,are MnSO₄.H₂O and AICl₃ and 0.41208 and -0.47819 respectively.For the organic substances,the first principal component represent folacin and biotin,and there weight coefficient are all 0.60015,and the second principal component represent myoinositol and thiamine,and there weight coefficient are 0.53281 and 0.53052 respectively.2.The chemical stability in different storage conditions of NAA,IBA and KT solution were studied.The results showed that:(1) In any case tested,the 3 kinds plant growth regulator will decompose gradually in water solution when stored for more than 10 days.So, for the accuracy of experiments,their solution can only be used within 10 days;(2) Temperature and light conditions have no affect on the stability of NAA.But the dark condition can help keep the stability for IBA,and the lower temperature and dark conditions for KT;(3) Compared with water,in 95%alcohol KT,IBA and NAA can keep stability for longer time.Thus,if a long time of storage is need,the solutions of KT,IBA and NAA should be made with 95%alcohol.3.The Absorption effect of active charcoal for IAA,KT and 2,4-D used in tissue culture were studied.The results showed that:(1) The absorption ability of active charcoal for IAA and KT is much larger than for 2,4-D;(2) The absorption ability of different particle diameter of active charcoal for the 3 kinds of plant hormones is almost the same;(3) When the time of absorption longer than 12h,the amounts absorbed of all the 3 kinds plant hormones were increased obviously;(4) In a range studied,the amounts absorbed of all the 3 kinds plant hormones showed a significant linear correlation with the amounts of active charcoal used;(5) The sequence of active charcoal,plant hormones and MS medium ingredient added has no influence on the amounts absorbed of all the 3 kinds plant hormones.