| Object iveTo observe the effects of scalp acupuncture acting on patients with insomnia. At the same time, by animal experiment study, To explore the effects of Scalp acupuncture acting on insomnia rats after Intraperitoneal injection of para-chlorophenylalanine (PCPA), and the effects of the content of the Cytokine IL-1β、1L-6、TNF-α in the brains of rats after Acupuncture treatment。MethodsThis research is divided into two parts:clinical study and animal experimental study:(1) Clinical study60Patients with insomnia were assigned to the treatment group (Scalp acupuncture group) and control group (Drug group) according to the principle of simple random, control and blindness. There were30patients in the treatment group (Scalp acupuncture group) and30patients in the control group (Drug group)The specific treatment methods are as follows:Scalp acupuncture group:prescription:the main points:Baihui Sishencong,Shenting,Yintang.The supplementary points:Hyperactivity of fire due to yin deficiency:Fuliu, Neiguan, Sanyinjiao; Deficiency of the heart and spleen:Xinshu, Pishu, Geshu; Deficiency of the heart-qi and gallbladder-qi:Xinshu, Danshu, Geshu; Hyperactivity of fire due to liver-qi depression:Taichong, Fengchi, Fuliu; Phlegm-heat accumulation: Fenglong, Quchi, Neiting; Imblance of the stomach:Neiguan, Zusanli, Gongsun,, The supplementary points are on both sides. Retaining needle time:30min Once a day, rest on Sunday. Drug group:estazolam tablets, Made in Hubei province (H42021522), Purchased from Guangzhou University of Traditional Chinese Medicine, first affiliated Hospital outpatient service pharmacy, lmg a day, take it before sleep, or2mg a day when needed. One week is a course of treatment, A total of2weeks.After2weeks of treatment, all of the patients were evaluated through clinical symptoms, syndrome of TCM, sleep efficiency, PSQI.(2) Animal experimental studyEstablishing the animal model of insomnia by injecting para-chlorophenylalanine (PCPA) into the rats abdominal cavity. After rat model established, they were randomly divided into4groups:Blank control group (n=10), Model group(n=10), Scalp acupuncture group(n=10), Drug group(n=10).The specific treatment methods are as follows:A. Blank control group:Feeding rats in General environment. NO modeling, NO treating.B. Model group:Making rat model succefully. Fixed in the rat in homemade cage for15min every morning. NO treating. Once a day, total to6days.C. Drug group:Making rat model succefully. Estazolam tablets, Made in Hubei province (H42021522), Purchased from Guangzhou University of Traditional Chinese Medicine, first affiliated Hospital outpatient service pharmacy, lmg a day, Employing mouse dose of body surface area formula to calculate the dose in rats0.92mg/kg, Gavage it to rats during. Once a day, total to6days.D. Scalp acupuncture group:Making rat model succefully. Fixed in the rat homemade cage for15min every morning. Acupuncture prescription:Baihui, Yintang. Once a day, total to6days.During the experiment, the sleep behavioral of rat of every group were evaluated,the terms such as:Sleep duration, aggressive behaviour, Degree of excitement, Degree of movement during the activity, High spirits or burnout, Degree of quiet, Easy to wake up or not, Diet, Amount of business.The animals were decapitated8th day after made model, removing the brain tissue.cleaning, grinding, centrifugation, Extracting supernatant fluid, and ELISA method was used to detect the content of the Cytokine IL-1β、1L-6、TNF-α in the brains of rats after Acupuncture treatment。Results (1) The scores of clinical symptoms assessmentAfter the treatment,the scores of the two group was tested by Rank sum test, all the value of are lower than that before the treatment, except the total scores. Compared between the two group, the value of P of the items such as sleep latency, duration, number of night waking, dreaming are of no significant difference (P>0.05), while the value of P of the items such as the state after wake up, sleep evaluation, total scores are of significant difference (P<0.01), it show us thatrscalp acupuncture therapy is superior to drug therapy in improving symptoms.(2) The scores of syndrome of TCM assessmentAfter the treatment, the scores of the two group was tested by Rank sum test, all the value of are lower than that before the treatment, except the total scores. Compared between the two group, the value of P of the items such as difficulty in falling asleep, more than a dream, Palpitations easy to panic, sweating, difficulty in concentrating, dry throat are of no significant difference (P>0.05), while the value of P of the items such as Early morning sleepiness, fatigue limbs, forgetfulness, irritability total scores are of significant difference (P<0.01), it show us that:scalp acupuncture therapy is superior to drug therapy in improving symptoms.(3) The scores of PSQI assessmentAfter the treatment, the scores of the two group was tested by Rank sum test, all the value of are lower than that before the treatment, except the total scores. Compared between the two group, the value of P of the items such as sleep time, sleep efficiency are of no significant difference (P>0.05), while the value of P of the items such as sleep quality, time of falling asleep, sleep disorders, hypnotic drugs, daytime dysfunction, total scores are of significant difference (P<0.01), it show us that:scalp acupuncture therapy is superior to drug therapy in improving symptoms.(4) Clinical effective ComparisonComparing two groups of clinical curative effect. Result:in Scalp acupuncture group,18cases(60.00%) were healed,6cases (20.00%) were significant effective,4cases(13.33%)were effective,2cases (6.67%) were invalid. The effective rate is93.33%. In body acupuncture group,8cases(26.67%) were healed,9cases (30.00%) were significant effective,7cases(23.33%)were effective,6cases (20.00%) were invalid. The effective rate is80%. By statistical analysis, the difference petween two groups is of statistical significance (P<0.05). Conclusion:Scalp acupuncture is superior to treat patients with insomnia than drug therapy.(5) The content of the Cytokine IL-1β in the brains of ratsCompared with the blank group, model group, drug group through One-Way ANOVA, the content of the cytokine IL-1β in the brains of rats was obviously increased, the difference is of statistical significance (P<0.01), it show us that scalp therapy can increase the content of IL-1β.(6) The content of the Cytokine TNF-α in the brains of ratsCompared with the blank group, model group, drug group through One-Way ANOVA, the content of the cytokine TNF-α in the brains of rats was obviously increased, the difference is of statistical significance (P<0.01), it show us that scalp therapy can increase the content of TNF-α.(7) The content of the Cytokine1L-6in the brains of ratsCompared with the blank group, model group, drug group through One-Way ANOVA,the content of the cytokine1L-6in the brains of rats was obviously increased, the difference is of statistical significance (P<0.01), it show us that scalp therapy can increase the content of1L-6.Conclusion(1) The two therapy both can improve the insomnia, however, The efficacy of scalp acupuncture is more significant than the efficacy of body acupuncture in improving the state after wake up, sleep evaluation, Early morning sleepiness, fatigue limbs, forgetfulness, irritability, sleep quality, time of falling asleep, sleep disorders, hypnotic drugs, daytime dysfunction, total scores.(2)The two acupuncture therapies can promote the content of the cytokine IL-1β、1L-6、TNF-α in the brains of rats. But the efficacy of scalp acupuncture is more significant than body acupuncture. And the model group recovered automatic in some extent. |
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