Research On Molecular Computing System Based On Logical Circuits

With the continuous development of science and technology,people ’s demand for computer performance improvement is becoming more and more urgent.Traditional silicon-based computers have reached the development limit,and it is difficult to achieve a greater improvement.As a biological material,DNA has the advantages of small size and good biological properties,so scientists have focused on DNA,and using DNA to design logic circuits for biological computing has become one of the areas that many scientists are keen to study.DNA computing has a long history of development,as early as 1994,Professor Adleman of the United States proposed the concept of DNA computing to solve Hamilton path problems,and finally achieved remarkable results.In 2004,Japanese scientists combined digital circuits in the traditional electronic field with DNA computing,and successfully constructed DNA logic circuits.So far,the door to the world of DNA computing has been opened.In the paper,a novel DNA strand displacement technology,namely allosteric DNA strand displacement technology,is proposed by combining the frontier hot technology and theory-DNA strand displacement technology and allosteric effect of protein in the field of biological computing.Based on this technology,DNA logic gates and logic circuits are constructed,and the possibility of realizing molecular computing system on the basis of allosteric DNA strand displacement technology is deeply explored.The paper has carried out multi-level research such as simulation and experimental verification.The first is the design and implementation of DNA strand displacement technology based on allosteric effect.By combining the allosteric effect of protein molecules with the traditional DNA strand displacement technology,a DNA strand displacement model based on allosteric effect is designed.The principle is to identify the allosteric sites in the DNA complex by inputting a DNA trigger strand.The conformational change will report the signal output;The second is the design and implementation of logic gates based on allosteric strand displacement.Based on the above DNA allosteric strand displacement technology,in order to explore its more possibilities in the field of biocomputing,this paper introduces it into simple molecular logic gates.The specific principle is to input the DNA trigger strand,activate the allosteric strand displacement logic unit by identifying the allosteric site for calculation,and finally obtain the operation result through the released report signal.In this paper,three molecular logic gates of YES,AND and OR are designed and implemented respectively.Thirdly,the design and implementation of parallel circuit and cascade circuit based on allosteric strand displacement.Based on the successful construction of logic gates based on allosteric strand displacement,in order to further study the possibility of building a complex information processing logic system model,this paper introduces it into parallel circuits and four-layer cascade circuit.The specific principle is to construct a multi-individual strand displacement module,the parallel circuit without interference and the four-level circuit in which information can be transmitted layer by layer are realized.The novel DNA strand displacement technology proposed in this paper will provide new ideas and solutions for the practical application of DNA logic circuits in the field of molecular computing.