Design And Implementation Of Multi-input Complex Logic Circuit Based On DNA Strand Displacement

In recent years,DNA molecular computing,with its powerful information storage capacity and parallel computing capabilities,has become the focus of researchers.DNA strand displacement technology is a novel type of calculation method,which is a dynamic nanotechnology improved by researchers on the basis of self-assembly technology.Compared with DNA self-assembly technology,it can realize the dynamic connection of signals between adjacent modules,which is a new method for constructing logic gates and logic circuits.In this paper,series of molecular logic circuits are designed and studied based on the principle of DNA strand replacement,the main contents are as follows:Aiming at the problems of large amount of calculation and slow operation speed at traditional factorial operation,a four-input factorial addition circuit is designed and implemented based on DNA strand displacement technology.This design is built on the basis of factorial operation.The construction of digital logic circuit and the analysis of simulation results show that the reliability of logic circuit designed is verified.It provides a novel idea for building higher order factorial circuits in the future.At the same time,this circuit can also be extended to other mathematical operation circuits.Aiming at the problems of low input signal and difficult cascading of traditional logic circuits,a complex logic circuit with three inputs and nine outputs,based on DNA strand displacement,is constructed.Combined with the thought of dual-track train and the structure of seesaw gate,the biochemical logic circuit is built.Next,the reliability of the designed circuit is verified by compiling simulation codes for simulation experiment.The design of this logic circuit also shows that DNA strand displacement technology can be extended to input and output circuits with larger scale.Aiming at the difficulty of multifunctional logic circuit design,a four-input multilayer logic circuit is designed and implemented.Each of these modules can implement multiple logic operations,and the other three inputs and AND and OR logic combination of the calculation mode are realized by controlling the input of each logical gate.In this paper,the reliability of the design is verified by a series of chain displacement reaction processes and experiments with simulation software.The idea of this circuit is expected to be extended to the design of more complex multilayer logic circuits.In this paper,the multi-input logic circuit is designed based on DNA strand displacement technology,the complexity of which are increasing from simple to complex,and from single-layer to multi-layer scale.The circuits designed can be expanded to multi-input and multi-cascade molecular circuits.In this paper,the design not only provides novel ideas for constructing multi-bit and multi-function logic circuits,but also provides a reference for building large-scale biological integrated circuits in the future.