| Objective: To explore the intervention effect of Yiqi Runchang Formula on rats with qi deficiency constipation,and to explore the mechanism of Yiqi Runchang Formula on constipation based on VIP-c AMP-PKA-AQP3 pathway,in order to provide evidence for the treatment of constipation with traditional Chinese medicine.Methods: 48 SD rats were randomly divided into 6 groups: blank group,model group,lactulose group,low dose group of Yiqi Runchang Formula,middle dose group of Yiqi Runchang Formula,and high dose group of Yiqi Runchang Formula,with 8 rats in each group.The blank group was given normal saline by gavage every day,and the other five groups were given loperamide suspension(3mg/kg)by gavage every day and fasting every other day for two weeks to prepare the animal model of qi deficiency constipation,and then the model was evaluated.After the establishment of the model,the blank group and the model group were given normal saline by gavage every day,the lactulose group was given lactulose oral liquid(1.67g/kg)by gavage every day,and the high,middle and low dose groups of Yiqi Runchang Formula were given Yiqi Runchang Chinese medicine(17.5,8.75,4.375g/kg)by gavage every day for two weeks.After two weeks,collect rat feces,weigh and calculate the weight,number of particles and moisture content of rat feces,kill the rats,and collect colon tissue.HE staining was used to observe the pathological changes of the colon,q PCR was used to detect the m RNA expression level of VIP,c AMP,PKA and AQP3 in the colon of rats,and western blot was used to detect the protein expression level of VIP and AQP3 in the colon.Results: 1.After modeling,compared with the blank group,the number of feces particles and the weight of feces in the model group were significantly reduced(P<0.01).2.After drug intervention,compared with the blank group,Yiqi Runchang Formula can significantly increase the weight and number of feces of constipation rats,improve the physical condition of constipation rats,and increase the weight of constipation rats,but there is no significant difference in the water content of feces(P>0.05).Compared with the blank group,the weight of rats in the model group decreased significantly(P;Compared with the model group,the number of defecation particles in the high dose group of Yiqi Runchang Formula increased significantly(P 05);Compared with the model group,the dry weight of feces of rats in the middle and high dose groups of Yiqi Runchang Formula increased significantly(P 05);Compared with the model group,there was no significant change in the fecal water content of rats in the low,middle and high dose groups of Yiqi Runchang Formula(P>0.05).Compared with the lactulose group,there was no significant difference in the body weight of rats in the low,middle and high dose groups of Yiqi Runchang Formula(P>0.05).The wet weight of feces in the middle and high dose groups increased significantly,with a statistically significant difference(P<0.05).The number of fecal particles increased,with a statistically significant difference(P<0.05);HE staining results showed that Yiqi Runchang Formula could significantly improve the colon morphology of constipation rats,increase the number of goblet cells,and improve the infiltration of inflammatory cells;The q PCR results showed that the m RNA levels of VIP,c AMP,PKA and AQP3 in the model group were significantly lower than those in the blank group(P;Compared with the blank group,the protein expression of VIP and AQP3 in the colon tissue of the model group was significantly decreased(P;Compared with lactulose,Yiqi Runchang Formula has a stronger ability to improve AQP3 protein expression in a dose-dependent manner.The high-dose group of Yiqi Runchang Formula can significantly increase AQP3 protein expression.Conclusion: Yiqi Runchang Formula can significantly improve the general condition and constipation state of rats with qi deficiency constipation,increase the number and weight of stool,and the improvement is most obvious in the high-dose group of Yiqi Runchang Formula.Yiqi Runchang Formula can effectively improve the constipation of rats with qi deficiency constipation by regulating the VIP-c AMP-PKA-AQP3 pathway,further revealing the partial mechanism of the treatment of constipation by traditional Chinese medicine,and providing some ideas and references for the clinical application of traditional Chinese medicine. |
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